ExpressPlex™ 2.0 Library Prep Kit
ExpressPlex 2.0: The workflow simplicity of one-step library prep combined with the performance of TnX for high throughput sequencing of plasmids, amplicons and microbial whole genomes.
- Improved performance: TnX transposase for reduced bias and improved coverage uniformity
- Ease of use: Fragmentation, barcoding, and amplification in a single step
- Rapid: 100-minute workflow (30 minutes or less hands-on) with pre-plated, reaction-ready reagents
- Flexible: 96- or 384-well plate format
- Scalable: High-level sample multiplexing (up to 6144) and auto normalization
We’ve unleashed TnX, our next generation transposase, in ExpressPlex 2.0 – a library prep kit that changes your NGS workflow forever!
ExpressPlex 2.0 is an automation-friendly, one-step library prep powered by TnX to reduce bias and improve coverage uniformity using a highly streamlined workflow for sequencing synthetic constructs like plasmids & amplicons as well as microbial WGS.
ExpressPlex 2.0 One-step Library Prep (96-well format)
ExpressPlex 2.0 One-step Library Prep (384-well format)
“Anyone can rapidly produce a quality NGS library using ExpressPlex 2.0!”
– Zachary Neuschaefer, Principal NGS Engineer, LifeMine Therapeutics
TnX Transposase: Quality Libraries for Quality Sequencing
ExpressPlex 2.0 uses tagmentation powered by TnX, the next generation transposase, to simultaneously fragment & index sample DNA. Average fragment size ranges from 400-1200 bp.
Typical fragment profile following analysis on an Agilent TapeStation System. Data shown are for a 96-plex sample.
TnX: Transposase Engineered for Reduced Insertion Bias
Better transposase = better sequencing. Take your plasmid and amplicon screening to the next level!
ExpressPlex 2.0 is the only library prep kit powered by TnX! TnX was engineered to reduce insertional bias to improve uniformity of coverage. When directly compared to a Tn5-based competitor library prep kit for sequencing of 2 different plasmids, ExpressPlex 2.0 produced significantly lower CVs and range of coverage (max/min).
Library preparation for pUC19 and pMal-c67 was conducted using ExpressPlex 2.0 kit and a Tn5-based competitor kit using standard manufacturers’ protocols. Libraries were sequenced using an Illumina MiSeq.
Product Highlights
Auto-Normalization of Read Depth
ExpressPlex demonstrates significantly higher levels of normalization compared to competitors, enabling a simplified workflow where individual normalization is no longer required to achieve more consistent read-depths across samples.
Libraries were prepared from three different reference plasmids of varying sizes across a 10-fold input range at full reaction volume for ExpressPlex (16 µL) and Nextera XT (50 µL), as well as at ¼ miniaturized reaction volume for Nextera XT.
For each method, inputs ranged from 4-40ng for ExpressPlex (standard input is 16ng) and 0.4 – 4ng for Nextera XT (standard input is 1ng). Inputs were scaled appropriately for 1/4 reactions.
Auto-Normalization of Insert Size
ExpressPlex generates highly consistent library insert sizes over a broad range of DNA inputs.
Libraries from pUC19 plasmid DNA were simultaneously prepared at 3 different input amounts (n=8 each input amount). ExpressPlex demonstrated greater consistency in average insert size across a 10-fold range. In each case, the standard input for both kits (16ng for ExpressPlex, 1ng for Nextera XT) was also included as a mid-range data point.
POSTER: One-step Library Preparation Method Supporting Skim-seq, Low Pass Sequencing Approach for Genotype Imputation
Recorded Webinar | Octant Bio
The game-changing capabilities enabled by the ExpressPlex workflow were highlighted in a webinar collaboration with the synthetic biology team from Octant Bio, a drug discovery company located in Emeryville, CA. Octant’s Henry Chan, PhD (Synthetic Biology Lead) and Bryan Jiang (Research Associate) discussed the application of ExpressPlex to OCTOPUS, the high-throughput plasmid sequencing platform that powers Octant’s therapeutic discovery platform. Watch the video to discover how ExpressPlex has dramatically improved OCTOPUS and accelerated their overall discovery process.
Improving Sustainability Through Streamlined Protocols
The automation-friendly ExpressPlex one-step tagmentation workflow enables a >80% reduction in tips/plastics usage. We help drive sustainability not only in our internal sequencing services pipeline, but for our customers across the globe.
Listen to an interview with Jack Leonard, seqWell’s co-founder and CTO, as he discusses addressing increased sustainability within the world of synthetic biology on sptlabtech’s “labtech unplugged” podcast.
Are you in need of bulk-dispensed product for your miniaturized reactions?
ExpressPlex 2.0 Specifications
| ExpressPlex 2.0 Specifications | |
| Primary Applications | Plasmid & amplicon sequencing, and microbial whole genome sequencing (WGS) |
| Sample Input types | Purified plasmid DNA, RCA-amplified DNA, amplicons >350bp, colony PCR amplicons, microbial genomic DNA |
| Transposase | TnX – Next generation engineered transposase |
| Kit format |
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| DNA Input Recommended | 96-well format:
384-well format:
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| Total Library Prep Time | 100 minutes (30 minutes hands-on time) |
| Indexing Method | Combinatorial Dual Indexing |
| Number of Unique Index Combinations | Up to 6144 |
| Batch Size |
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| Output Fragment Range* | 400 – 1,200bp |
| Number of PCR Cycles |
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| Sequencer Compatibility |
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* Fragment size will depend on magnetic bead cleanup ratios used.
ExpressPlex 2.0 Library Prep Kit Includes:
- Indexing Reaction Plate(s)
- Ready Reaction Mix Plate(s)
- MAGwise Paramagnetic Beads
- Users do not need to supply polymerase, primers, or barcodes. These are contained within the supplied ready reaction mix.