Realize the potential of PacBio long read sequencing using seqWell’s LongPlex™ Long Fragment Multiplexing Kit that rapidly multiplexes samples for increased sequencing throughput and cost-effectiveness.
Realize the throughput and cost-effectiveness of PacBio HiFi sequencing using seqWell’s LongPlex Long Fragment Multiplexing Kit. Its speed, simplicity, and scalability enable massive sample multiplexing that unleashes scalable long read sequencing.
Enzymatic fragmentation using adapter-loaded transposase allows for simultaneous fragmentation and indexing of gDNA. Importantly, this eliminates the need for mechanical shearing of gDNA and greatly increases sample throughput.
Efficiently multiplex more samples per flow cell without compromising sequencing quality on PacBio sequencing platforms. To run 384 long read samples, you no longer need to perform 384 PacBio SMRTbell library preparations! Simply use the plate-based LongPlex method, followed by 4 SMRTbell library preps enabling 384 sample on a single flow cell. Pooling samples early allows you to maximize sequencer throughput while driving down costs.
Specs | Description |
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Primary Applications | Long read sequencing for:
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Sample Types* | Genomic DNA (DIN ≥8.0 recommended) |
Reactions per Kit | 96 reactions |
DNA Input Recommended | 250 – 500ng |
Indexing Method | Combinatorial Dual Indexing |
Number of Unique Index Combinations | 96 |
Batch Size** | 1-24 samples |
Output Fragment Range*** | 5,000 – 8,500bp |
PCR Amplification |
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Total Protocol Time |
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Sequencer Compatibility | PacBio Revio
PacBio Sequel II & IIe |
*Other sample types may be compatible. Contact seqWell’s support for guidance. **Up to 96 samples can be pooled past LongPlex barcoding prior to PacBio SMRTbell but it may impact read output and coverage uniformity ***Fragment size will depend on magnetic bead cleanup ratios used