seqWell’s integrated normalizing library prep technology allows the creation of balanced library pools without the need for sample or library normalization. Our fast, simplified workflows multiplex 100’s to 1000’s of samples for loading on a single sequencing run enabling enhanced overall sequencing performance.
Multiplex 1000’s of samples with ease using our kits.
Built-in normalization with every kit.
Uniform insert sizes & sample read counts.
Improved overall sequencing performance.
P7 primers and unique i7 indexes are inserted randomly into each DNA sample by a transposon. The same amount of P7 is added to each sample regardless of DNA input amount. This limiting reagent normalizes the samples, which are now pooled into a single tube.
The pool-specific i5 barcoded adapters containing P5-adapters are now added in the second transposition reaction. Excess pool barcoding reagent inserts the same average distance from every sample barcode.
The final step is a library amplification with universal primers and final SPRI purification. The pooled library is now ready to be loaded in an Illumina® sequencer to produce normalized or balanced distribution of sequencing reads.
“The integrated auto-normalization in the plexWell kit allows us to treat 96 samples as 1—one Qubit measurement to get the average concentration of the input plate, one tube to process in the library prep, and one library pool to normalize and load onto the iSeq. This is a huge advantage of the plexWell kit.” – Areta Buness
At left, sequencing results obtained for sequencing 192 samples of amplified single-cell cDNA with plexWell™ (blue) and For Nextera™ reagents (gray). Input DNA was pre-normalized, whereas plexWell™ library was made from un-normalized amplified cDNA. Read count variation for plexWell™ showed 27% CV versus 71% CV for Nextera™.