Technology
Discover the Features and Benefits of “True Multiplexing”
seqWell’s integrated normalizing library prep technology allows the creation of balanced library pools without the need for sample or library normalization. Our fast, simplified workflows multiplex 100’s to 1000’s of samples for loading on a single sequencing run enabling enhanced overall sequencing performance.
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Simple & Fast
Multiplex 1000’s of samples with ease using our kits.
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Normalization
Built-in normalization with every kit.
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Performance
Uniform insert sizes & sample read counts.
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Quality
Improved overall sequencing performance.
Product Highlights
- Multiplex 100’s to 1000’s of samples in a single sequencing run
- Eliminate time-consuming library quant
- Remove the need for tedious input normalization
- Uniform depth of coverage and insert size on all samples
- No upfront or additional equipment needed
Highlighted Sequencing Applications
Plasmid and Amplicon Sequencing Gene Editing QC Low-pass Whole Genome Sequencing Microbial Sequencing Viral Sequencing
Simple Three Step Protocol:
Our NGS workflows utilize a transposase to selectively tag DNA samples in a unique sequential manner. The sequential tagging process provides more control of each step versus other transposon methods and less prone to bias and input DNA variations.
Sample Barcoding
Unfragmented DNA is tagged with sample-specific i7-barcoded adapters.
P7 primers and unique i7 indexes are inserted randomly into each DNA sample by a transposon. The same amount of P7 is added to each sample regardless of DNA input amount. This limiting reagent normalizes the samples, which are now pooled into a single tube.
Pool Barcoding
Pooled sample-barcoded DNA is tagged with pool-specific i5-barcoded adapters.
The pool-specific i5 barcoded adapters containing P5-adapters are now added in the second transposition reaction. Excess pool barcoding reagent inserts the same average distance from every sample barcode.
Amp + Purification
Barcoded library fragments are amplified.
The final step is a library amplification with universal primers and final SPRI purification. The pooled library is now ready to be loaded in an Illumina® sequencer to produce normalized or balanced distribution of sequencing reads.
Check out what our customers are saying:
“The integrated auto-normalization in the plexWell kit allows us to treat 96 samples as 1—one Qubit measurement to get the average concentration of the input plate, one tube to process in the library prep, and one library pool to normalize and load onto the iSeq. This is a huge advantage of the plexWell kit.” – Areta Buness
Simple & Scalable Workflow:
Integrated Normalization = More Samples Per Sequencing Run
Read Count Normalization Variable Input
Compression of Input DNA Range
plexWell™ Achieves a Significantly Better Level of Multiplexing Uniformity for Highly Multiplex Sequencing Applications.
At left, sequencing results obtained for sequencing 192 samples of amplified single-cell cDNA with plexWell™ (blue) and For Nextera™ reagents (gray). Input DNA was pre-normalized, whereas plexWell™ library was made from un-normalized amplified cDNA. Read count variation for plexWell™ showed 27% CV versus 71% CV for Nextera™.
Precision Multiplexing
seqWell technology yields balanced multiplexed libraries containing highly uniform insert size distributions and samples read counts.
Median Insert Size Distribution for 96-plex Library:
plexWell™ Insert Size Distribution:
plexWell™ Delivers Minimal Bias for Diverse Applications
Microbial Genome
Mammalian Genome
Order seqWell™ Library Prep Kits
Our library prep kits are available for all your sequencing applications. Feel free to browse our seqWell products.