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plexWell’s integrated normalizing library prep technology allows the creation of balanced library pools without the need for sample or library normalization. The simplified 3-hour workflow multiplexes 100’s to 1000’s of samples for loading on a single sequencing run enabling enhanced overall sequencing performance.
Multiplex 1000’s of samples with ease using our kits.
Built-in normalization with every kit.
Uniform insert sizes & sample read counts.
Improved overall sequencing performance.
P7 primers and unique i7 indexes are inserted randomly into each DNA sample by a transposon. The same amount of P7 is added to each sample regardless of DNA input amount. This limiting reagent normalizes the samples, which are now pooled into a single tube.
The pool-specific i5 barcoded adapters containing P5-adapters are now added in the second transposition reaction. Excess pool barcoding reagent inserts the same average distance from every sample barcode.
The final step is a library amplification with universal primers and final SPRI purification. The pooled library is now ready to be loaded in an Illumina® sequencer to produce normalized or balanced distribution of sequencing reads.
At left, sequencing results obtained for sequencing 192 samples of amplified single-cell cDNA with plexWell™ (blue) and For Nextera™ reagents (gray). Input DNA was pre-normalized, whereas plexWell™ library was made from un-normalized amplified cDNA. Read count variation for plexWell™ showed 27% CV versus 71% CV for Nextera™.