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Technology

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Discover the Features and Benefits of “True Multiplexing”

plexWell’s integrated normalizing library prep technology allows the creation of balanced library pools without the need for sample or library normalization. The simplified 3-hour workflow multiplexes 100’s to 1000’s of samples for loading on a single sequencing run enabling enhanced overall sequencing performance.

  • Simple & Fast

    Multiplex 1000’s of samples with ease using our kits.

  • Normalization

    Built-in normalization with every kit.

  • Performance

    Uniform insert sizes & sample read counts.

  • Quality

    Improved overall sequencing performance.

Product Highlights

  • Multiplex 100’s to 1000’s of Samples in a Single Sequencing Run
  • Eliminate Time-Consuming Input and Library Quant
  • Uniform Depth of Coverage and Insert Size on All Samples
  • No Upfront or Additional Equipment Needed

Highlighted Sequencing Applications

Microbial Sequencing Low-pass Whole Genome scRNA-Seq Plasmid Sequencing

Simple Three Step Protocol:

plexWell™ utilizes a transposase to selective tag DNA samples in a unique sequential manner. The sequential tagging process provides more control of each step versus other transposon methods and less prone to bias and input DNA variations.

Sample Barcoding

Unfragmented DNA is tagged with sample-specific i7-barcoded adapters.

P7 primers and unique i7 indexes are inserted randomly into each DNA sample by a transposon. The same amount of P7 is added to each sample regardless of DNA input amount. This limiting reagent normalizes the samples, which are now pooled into a single tube.

Pool Barcoding

Pooled sample-barcoded DNA is tagged with pool-specific i5-barcoded adapters.

The pool-specific i5 barcoded adapters containing P5-adapters are now added in the second transposition reaction. Excess pool barcoding reagent inserts the same average distance from every sample barcode.

Amp + Purification

 Barcoded library fragments are amplified.

The final step is a library amplification with universal primers and final SPRI purification. The pooled library is now ready to be loaded in an Illumina® sequencer to produce normalized or balanced distribution of sequencing reads.

Check out the plexWell workflow in action:

Simple & Scaleable Workflow:

Integrated Normalization = More Samples Per Sequencing Run

Read Count Normalization Variable Input

Compression of Input DNA Range

plexWell multiplexed libraries vs Nextera

plexWell™ Achieves a Significantly Better Level of Multiplexing Uniformity for Highly Multiplex Sequencing Applications.

At left, sequencing results obtained for sequencing 192 samples of amplified single-cell cDNA with plexWell™ (blue) and For Nextera™ reagents (gray). Input DNA was pre-normalized, whereas plexWell™ library was made from un-normalized amplified cDNA. Read count variation for plexWell™ showed 27% CV versus 71% CV for Nextera™.

Precision Multiplexing

plexWell technology yields balanced multiplexed libraries containing highly uniform insert size distributions and samples read counts.

Median Insert Size Distribution for 96-plex Library:

plexWell™ Insert Size Distribution:

plexWell™ Delivers Minimal Bias for Diverse Applications

Microbial Genome

Mammalian Genome

Order plexWell™ Library Prep Kits

plexWell™ library prep kits are available for all your sequencing applications. Feel free to browse our plexWell™ products.