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Plasmids and PCR products are the bread and butter of molecular biology labs the world over.

Broad-based access to cost-effective, next-generation sequencing (NGS) and advances in synthetic biology have revolutionized the scale on which plasmids can be constructed, screened, and manipulated for wide-ranging applications, including biomarker discovery, antibody engineering, genome editing, and gene therapy. However, NGS library preparation throughput, performance, and cost remain bottlenecks in high-throughput plasmid sequencing.

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Learn how seqWell’s multiplexed plexWell™ technology enables robust, high-throughput plasmid verification and screening in both research and commercial settings.

Scalable, true multiplexed library preparation enables high-performance, high-throughput plasmid sequencing

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Read distributions by plate and well position for 960 plasmids sequencing in a single Illumina® MiSeq™ (2 x 250 bp) run. Samples were processed in 10 plates of 96 plasmids each with the plexWell 384 Library Preparation Kit. Assemblies were obtained for 955 of the 960 plasmids (99.5%), and were free of ambiguities for 99.2% of the samples.

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For more information on high-throughput synthetic construct sequencing, please contact us to work with one of our product specialists.

Plasmid Sequencing Resources

Read these blogs to find out how the plexWell™ technology enables Addgene to optimize the QC verification and validation of thousands of plasmids per year.

The Power Behind NGS Plasmid Validation – seqWell

Addgene Moves to NGS Verification Powered by seqWell

 

Download our Application Note to learn about our de novo plasmid assembly workflow, library preparation and sequencing data quality, and comparative performance vs. other plasmid sequencing workflows.

Scalable, true multiplexed library preparation enables high-performance, high-throughput plasmid sequencing

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Caught in the Act: Tracking the Emergence and Divergence of SARS-CoV-2 through Statewide Testing and SequencingUPCOMING WEBINAR

Date:  March 23, 2022
Time: 11:00am (PDT),  2:00pm (EDT), 8:00pm (CEDT)

Learning Objectives

  • Understand the utility of full-genome viral sequence analysis for public health
  • Recognize how viral load impacts the ability to detect and sequence intact SARS-CoV-2
  • Identify the similarities and differences in host responses to SARS-CoV-2 depending on strain and immunologic history

Speakers

Frank A. Middleton, Ph.D.
Professor Director, SUNY Molecular Analysis CoreState University of New York (SUNY) Upstate Medical University

Joseph C. Mellor, Ph.D.
Co-founder and Chief Scientific Officer of seqWell Inc.