Plasmid Sequencing with plexWell™
Plasmids and PCR products are the bread and butter of molecular biology labs the world over.
Broad-based access to cost-effective, next-generation sequencing (NGS) and advances in synthetic biology have revolutionized the scale on which plasmids can be constructed, screened, and manipulated for wide-ranging applications, including biomarker discovery, antibody engineering, genome editing, and gene therapy. However, NGS library preparation throughput, performance, and cost remain bottlenecks in high-throughput plasmid sequencing.
Download our Application Note to learn how seqWell’s multiplexed plexWell™ technology enables robust, high-throughput plasmid verification and screening in both research and commercial settings.
Read distributions by plate and well position for 960 plasmids sequencing in a single Illumina® MiSeq™ (2 x 250 bp) run. Samples were processed in 10 plates of 96 plasmids each with the plexWell 384 Library Preparation Kit. Assemblies were obtained for 955 of the 960 plasmids (99.5%), and were free of ambiguities for 99.2% of the samples.
Looking for more information?
For more information on high-throughput synthetic construct sequencing, please email us at [email protected] to work with one of our product specialists.
Plasmid Sequencing Resources
Read these blogs to find out how the plexWell™ technology enables Addgene to optimize the QC verification and validation of thousands of plasmids per year.
The Power Behind NGS Plasmid Validation – seqWell
Addgene Moves to NGS Verification Powered by seqWell
Download our Application Note to learn about our de novo plasmid assembly workflow, library preparation and sequencing data quality, and comparative performance vs. other plasmid sequencing workflows.