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Current technology, though, is limited by the number of samples sequenced simultaneously (multiplexing), sensitivity, and turnaround time. plexWell™ technology combines a simple and efficient workflow with high sensitivity and unparalleled scalability that is ideal for high-throughput SARS-CoV-2 sequencing.
The ARTIC network provides protocols, primer sets, protocols, datasets and tutorials that enable research groups to sequence viruses, including the novel human coronavirus SARS-CoV-2. Scientists at seqWell used a modified ARTIC SARS-CoV-2 protocol and the plexWell 384 library prep kit to sequence viral samples, demonstrating the practical application of plexWell for COVID-19 research. You can read more about ARTIC protocol here.
SARS-CoV-2 viral load varies widely in nasal swab samples that are positive for COVID-19, and plexWell assays have the sensitivity to work with all but the most dilute virus-positive samples. Total nucleic acid isolated from saliva was used as the input, with the number of input viral genome copies similar to what is found in a typical nasal swab sample (~4.2 x 104 viral copies). Following RT and multiplex PCR, libraries are prepared using the plexWell kit. The libraries were then sequenced to produce full-length SARS-CoV-2 sequences.
The plexWell library prep kit delivers uniform and high sequence coverage for even low-input SARS-CoV-2-positive samples. Even at low copy numbers (160 copies), plexWell delivered 400X coverage with strong uniformity.
The plexWell library prep kit produces libraries with high mapping rates even for low-copy number SARS-CoV-2 samples.