Click here to check out the new plexWell™ SARS-CoV-2 Sequencing application page and get started today with trial kit discounts (50% off).

 

 

 

Simple workflow

ARTIC-compatible with no upstream purification

High throughput

Multiplex thousands of samples

Uniform Coverage

Down to 160 viral copies

Try plexWell library prep for
SARS-CoV-2 sequencing

We need to understand the SARS-CoV-2 virus now, and NGS provides a scalable approach to quickly analyze thousands of specimens.

Current technology, though, is limited by the number of samples sequenced simultaneously (multiplexing), sensitivity, and turnaround time. plexWell™ technology combines a simple and efficient workflow with high sensitivity and unparalleled scalability that is ideal for high-throughput SARS-CoV-2 sequencing.

Learn more about plexWell technology

Efficient and Highly Multiplexed Whole Viral
Genome Sequencing of SARS-CoV-2 Samples

Our new plexWell sample preparation method in combination with a widely-used ARTIC RT-PCR protocol greatly simplifies and increases the throughput of multiplex whole genome sequencing of SARS-CoV-2 RNA. It provides high quality results while reducing the time and cost of sequencing efforts. The method provides numerous advantages, including:

• robust performance
• simple workflow
• high multiplexing capacity

This cost-effective solution can provide up to 40% savings compared to other methods and reduce processing time from sample to result. The plexWell workflow is automation-friendly and compatible with a variety of platforms. plexWell enables sequencing of up to 1,152 samples in a single sequencing run and provides a simple workflow capable of meeting the high demand for COVID-19 testing.

Download the App Note

SARS-CoV-2 sequencing using plexWell

The ARTIC network provides protocols, primer sets, protocols, datasets and tutorials that enable research groups to sequence viruses, including the novel human coronavirus SARS-CoV-2. Scientists at seqWell used a modified ARTIC SARS-CoV-2 protocol and the plexWell 384 library prep kit to sequence viral samples, demonstrating the practical application of plexWell for COVID-19 research. You can read more about ARTIC protocol here.

Download the protocol

Streamlined workflow for SARS-CoV-2 whole-genome sequencing

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Viral load range in clinical SARS-CoV-2 clinical specimens

SARS-CoV-2 viral load varies widely in nasal swab samples that are positive for COVID-19, and plexWell assays have the sensitivity to work with all but the most dilute virus-positive samples. Total nucleic acid isolated from saliva was used as the input, with the number of input viral genome copies similar to what is found in a typical nasal swab sample (~4.2 x 104 viral copies). Following RT and multiplex PCR, libraries are prepared using the plexWell kit. The libraries were then sequenced to produce full-length SARS-CoV-2 sequences.

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Uniform and high SARS-CoV-2 coverage

The plexWell library prep kit delivers uniform and high sequence coverage for even low-input SARS-CoV-2-positive samples. Even at low copy numbers (160 copies), plexWell delivered 400X coverage with strong uniformity.

High SARS-CoV-2 accuracy

The plexWell library prep kit produces libraries with high mapping rates even for low-copy number SARS-CoV-2 samples.

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Learn more about how plexWell technology can maximize your SARS-CoV-2 sequencing effort.

Schedule a meeting today.