Tagify™ UMI Reagent Set
- SKU: 301200
$150.00 – $1,200.00
Tagify™ UMI reagents are designed to enable CRISPR or gene editing QC:
- Quickly and reliably fragment and tag genomic DNA via loaded Tn5 transposase
- Tagged sequence includes Illumina-compatible i5 barcode and unique molecular identifier (UMI) regions
- Useful for UDiTaS1 or RGen-Seq2 applications, CRISPR QC, and cell and gene editing QC
How Can Tagify UMI’s Help You?
“We use seqWell’s custom Tagify reagent for our UDiTaS process. The batches are consistent and provide similar tagmentation profiles and editing results. The reactions can be scaled from 96 to 384 wells, and we’ve been able to process thousands of reactions over one year.”
– Georgia Giannoukos, Ph.D., Director of Next Generation Sequencing, Editas Medicine
Recorded Webinar
Assessing CRISPR On-Target Editing and Structural Changes
In this webinar, Dr. Giannoukos highlighted how her team combined a custom Tagify reagent with their UDiTaS method to measure large deletions and inversions at the CEP290 editing site.
She also incorporated data demonstrating reproducibility of different Tagify batches. This webinar was transcribed into a blog if you prefer to read along.
Tagify UMI’s in Action
Multiplexed NGS-based QC Tools for Genome Editing Applications
Genome editing with technologies like CRISPR-Cas9 can be accompanied by off-target mutagenesis that are critical to measure and characterize. In general, it is effort-intensive, slow, and bias-prone to generate libraries of genomic DNA by fragmentation, size selection, end-repair and ligation prior to PCR. Library amplification is often followed by inefficient hybridization-based enrichment of sequences of interest to reduce sequencing cost.
In this poster, we describe the use of Tagify™ UMI Tn5 transposase-based reagents to circumvent these limitations.
Tagify is More Than Just UMI’s
Tn5 transposase is the centerpiece for seqWell’s work in building better NGS workflows.
Our Tagify toolbox utilizes the power of transposase methods to enable scalable throughput. We address the core issues that users face every day, like how to process more samples while saving time, money, and effort associated with getting the results you need.
Tagify can replace several distinct steps in a workflow and condense them into a single step that has equally high performance, a simpler workflow, and more predictable performance.
Applications include:
- Single-cell ATAC-Seq
- CRISPR edit verification (UDiTaS method)
- Cell/gene therapy QC
- Long-read sequencing technology fragmentation
Contact us for a custom-loaded Tagify solution with your sequence.
Recorded Webinar
Enabling Sequencing Applications with Improved Transposase-Based Solutions
The capacity and speed of modern DNA sequencing platforms has allowed sequencing to become an integral component of biomedical research and development pipelines. One key need emerging from this capability is for library tools that match sequencer technology with comparable workflow speed, throughput and efficiency.
In this video, we explore how transposase-based library technologies are well-suited for a number of challenging high-throughput workflows, and how innovations such as purePlex, ExpressPlex and Tagify are accelerating critical sequencing-based applications.
Tagify UMI Reagent Specifications
| Specs | Description |
|---|---|
| Primary Applications* | CRISPR or Gene Editing QC and Verification |
| Sample Types* | Genomic DNA |
| Reactions per Kit | Approximately 40 – Varies with Inputs and Application |
| DNA Input Recommended | 50ng |
| Number of Indices Included | 8 barcoded reagents are included |
| Barcoding Strategy |
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| Sequencer Compatibility | All Illumina sequencing platforms; Use with Element Biosciences AVITI™ or other sequencing platforms is possible with conversion kits for Illumina libraries |
1. UDiTaS Method: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861650/
2. RGEN-Seq Method: https://pubmed.ncbi.nlm.nih.gov/34880355/