In this webinar, Dr. Giannoukos highlighted how her team combined a custom Tagify reagent with their UDiTaS method to measure large deletions and inversions at the CEP290 editing site.
She also incorporated data demonstrating reproducibility of different Tagify batches. This webinar was transcribed into a blog if you prefer to read along.
Genome editing with technologies like CRISPR-Cas9 can be accompanied by off-target mutagenesis that are critical to measure and characterize. In general, it is effort-intensive, slow, and bias-prone to generate libraries of genomic DNA by fragmentation, size selection, end-repair and ligation prior to PCR. Library amplification is often followed by inefficient hybridization-based enrichment of sequences of interest to reduce sequencing cost.
In this poster, we describe the use of Tagify™ UMI Tn5 transposase-based reagents to circumvent these limitations.
Our Tagify toolbox utilizes the power of transposase methods to enable scalable throughput. We address the core issues that users face every day, like how to process more samples while saving time, money, and effort associated with getting the results you need.
Tagify can replace several distinct steps in a workflow and condense them into a single step that has equally high performance, a simpler workflow, and more predictable performance.
The capacity and speed of modern DNA sequencing platforms has allowed sequencing to become an integral component of biomedical research and development pipelines. One key need emerging from this capability is for library tools that match sequencer technology with comparable workflow speed, throughput and efficiency.
In this video, we explore how transposase-based library technologies are well-suited for a number of challenging high-throughput workflows, and how innovations such as purePlex, ExpressPlex and Tagify are accelerating critical sequencing-based applications.
|CRISPR or Gene Editing QC and Verification
|Reactions per Kit
|Approximately 40 – Varies with Inputs and Application
|DNA Input Recommended
|Number of Indices Included
|8 barcoded reagents are included
|All Illumina sequencing platforms;
Use with Element Biosciences AVITI™ or other sequencing platforms is possible with conversion kits for Illumina libraries
1. UDiTaS Method: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861650/
2. RGEN-Seq Method: https://pubmed.ncbi.nlm.nih.gov/34880355/