$165.00 – $3,000.00
Tagify™ i5 UMI Adapter-loaded Transposase reagents are for tagmentation-based NGS library preparation for simple, scalable, and reliable unique molecular identifier (UMI) addition. Accelerate your variant calling efforts with peace of mind.
To help you test out Tagify i5 UMI adapter-loaded transposase reagents for the first time, order now until the end of December 2024 and receive 50% off your first plate. This offer is intended for first time users and limited to a single 96 reaction Tagify i5 UMI plate.
In this webinar, Dr. Giannoukos highlighted how her team combined a custom Tagify reagent with their UDiTaS method to measure large deletions and inversions at the CEP290 editing site.
She also incorporated data demonstrating reproducibility of different Tagify batches. This webinar was transcribed into a blog if you prefer to read along.
Tagmenting DNA using Tagify i5 UMI loaded transposases requires a single 15-minute incubation at 55°C followed by reaction clean-up. Tagged DNA can then seamlessly integrate into downstream applications* such as gene editing assays like UDiTaS, RGEN, TTIS-seq, CHANGE-seq and others.
Specs | Description |
---|---|
Primary Applications | CRISPR or Gene Editing QC and Verification |
Sample Types | Genomic DNA |
Reactions per Kit |
|
Standard dispense volume per well | 3 microliters |
Transposase | Hyperactive Tn5 |
DNA Input Recommended | 50ng |
Number of unique barcodes | 24 or 96 unique molecular identifiers (UMI) |
Barcoding Strategy |
|
Output Fragment Range** (measured with Agilent DNA BioAnalyzer Chip) | 800 – 2000 bp (using region mode with a range of 200 – 7500 bp) |
Sequencer Compatibility | All Illumina sequencing platforms; Use with Element Biosciences AVITI™ or other sequencing platforms is possible with conversion kits for Illumina libraries |
**Because the tagged product in not a complete NGS library, sizing data and traces obtained from other methods, such as Agilent TapeStation or Femto Pulse, may vary.
Applications* – commercial use may require a license from a third party.
1. UDiTaS Method: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861650/
2. RGEN-Seq Method: https://pubmed.ncbi.nlm.nih.gov/34880355/