ExpressPlex™ Plus – Coming Soon
COMING SOON! ExpressPlex Plus: Workflow simplicity plus the performance of TnX for high throughput microbial applications like WGS and metagenomics. Go from extracted DNA to sequencer-ready libraries in 100 minutes!
- Microbial WGS, metagenomics & small genome WGS
- TnX brings reduced bias and improved coverage uniformity
- One-step fragmentation, barcoding, and amplification
- Auto-normalization
- Up to 95% fewer consumables
Coming Soon!
ExpressPlex Plus supports high-throughput microbial WGS & metagenomic sequencing applications through unmatched protocol simplicity, auto-normalization, large multiplexing capabilities, and lower all-in sequencing costs.
>> Powered by the 100-minute ExpressPlex workflow
>> Auto-normalization
>> Early sample pooling
>> Reaction-ready, 96- and 384-well plates
>> Indexes & purification beads provided
NGS workflow simplicity
Powered by the 100-minute ExpressPlex Workflow
One-step tagmentation & amplification, auto-normalization, and early pooling come together creating the simplest library prep workflow on the market. Don’t let library prep be a bottleneck!
Is Your NGS Workflow Holding You Back?
Blog Part 2: How the ExpressPlex Workflow Engineers Scalable Simplicity
Uniform sequence coverage across diverse genomes
ExpressPlex Plus achieves uniform coverage across yeast as well as bacteria of varying GC content and gram statuses.
Normalized, deduplicated coverage (y-axis) is shown for 3 microbial and 1 fungal species with (% GC from 32% to 66%) calculated using 1,000-base genomic windows (x-axis). The %CV of coverage is also shown.
Achieve higher mean coverages
Side-by side-comparison of mean coverage achieved vs. total PF reads required to meet that coverage. ExpressPlex Plus achieves higher mean coverage with less overall reads.
Methods: Library Prep Kit Comparison
- The ExpressPlex Plus library preparation kit was used to process eight bacterial strains in triplicate including gram positive and negative strains with GC contents between 29 – 66%. A yeast sample (S. cerevisiae) was also run.
- For comparison, two competitor kits were also run side-by-side with ExpressPlex Plus: an in-solution Tn5 based kit and a fragmentase plus normalization-by-ligation based kit.
- Starting DNA input for all was 30 ng of total genomic DNA.
- All libraries were sequenced on a NovaSeq X Plus (2 x 150 bp) with the goal of achieving ≥100x genome coverage per microbe.
- Following demultiplexing and alignment, assembly was done using SPAdes (v4.2.0) and Quast (v5.3.0)
Benchmarking One-Step Library Preparation Against Tn5-Based and Fragmentation–Ligation Workflows for Microbial Whole Genome Sequencing
Recover More Data From Your Sequencing
Simply spike-in PhiRx™ to increase your index color-balancing!
PhiRx Indexed Control NOW included with every ExpressPlex Plus Library Prep Kit
ExpressPlex Plus Specifications
| Primary Applications | Microbial/small genome WGS, Metagenomic sequencing |
| Sample Input types | Microbial or yeast genomic DNA |
| Transposase | TnX – Next generation engineered transposase |
| Kit format |
|
| DNA Input | Working range: 5-100 ng
Normalization range: 10-50 ng |
| Total Library Prep Time | 100 minutes (30 minutes hands-on time) |
| Indexing Method | Combinatorial Dual Indexing |
| Number of Unique Index Combinations | Up to 1536 |
| Batch Size | 8-96 samples |
ExpressPlex Plus Library Prep Kit Includes:
- Indexing Reaction Plate(s)
- Ready Reaction Mix Plate(s)
- MAGwise Paramagnetic Beads
- PhiRx Indexed Control
- Users do not need to supply polymerase, primers, or barcodes. These are contained within the supplied ready reaction mix.