Harnessing the Power of Transposases
seqWell innovation began in 2014 by harnessing the power of tagmentation – the simultaneous fragmenting and tagging (ligating NGS adapter sequences) of DNA – to replace traditional library preparation methods that use complex and lengthy protocols and to increase the power of multiplexing. One of our latest innovations is TnX, an engineered next-generation transposase and high-performance alternative to Tn5.
seqWell innovation doesn’t end with transposases. We harness their power using unique library prep workflows designed with simplicity & scalability in mind.
seqWell developed several simple, scalable NGS library preparation workflows — from one-step tagmentation & amplification, to seqWell directional tagmentation — that address a variety of needs.
- NGS system compatibility: Illumina, PacBio, Element Biosciences, Complete Genomics & Ultima Genomics
- Sequencing technology: Short-read and long-read sequencing
- Throughput: From 10’s to 1000’s of samples
- Sample types: Plasmids, amplicons, RCA, colony PCR, microbial and viral genomes & genomic DNA
- Kits to reagents: Ready-to-use kits and custom-loaded transposases
TnX… don’t be so biased
Engineered for Performance
TnX, seqWell’s next generation transposase and alternative to Tn5, was developed using fit-for-purpose engineering that targeted improvements in 3 key enzyme attributes: activity, insertion bias and robustness. These improved attributes translate into enhanced sequencing performance by improving NGS data quality and uniformity.
Better transposase, better libraries, better NGS
Reduced insertion site bias
Read start site insertion bias was measured by examining the frequency of bases in the first 9 bases of each read. Positions with higher per-base nucleotide bias are represented by heights for hyperactive Tn5 and TnX, and illustrate the reduced bias of TnX.
Products Powered by TnX
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Tagify Custom-loaded Transposase
TnX loaded with universal adapters, i5/i7 adapters, or customer-specified oligos – used for sensitive targeted gene editing QC analysis assays, as well as other custom assays.
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ExpressPlex 2.0
Ready-to-use, one-step library preparation kit for plasmid & amplicon sequencing.
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AgriPrep – powered by the ExpressPlex Workflow
Ready-to-use, one-step library preparation kit for high-throughput, low-pass sequencing of plant, animal, & human genomic DNA
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MosaiX
90-minute library prep using TnX-based directional tagmentation for production of high-performance libraries.
Blogs of Interest
PhiRx™: A Color-Balanced Spike-In Control for Improving Demultiplexing on XLEAP-SBS Illumina Sequencing Instruments
Short-Read Sequencing vs. Long-Read Sequencing: Which Technology is Right for Your Research?
Novel tagmentation-based workflow that improves throughput and scalability of long read assays
seqWell™ Library Preparation Kits and Reagents
Our library prep and multiplexing kits are available to support all your short and long-read sequencing applications. Contact us to find out which is right for you!