The seqWell Mission
Responsive to the needs of our customers, we engineer scalable genomics products that simplify sequencing workflows.
Why does seqWell focus on workflow simplicity & scalability? Because we understand that NGS is only as powerful as the workflow behind it. Without simplicity and scale, NGS workflows fundamentally limit scientific advancement: slower progress, fewer insights, and missed opportunities.
Library Prep: Unlocking NGS Potential
Library prep isn’t just a step—it’s an NGS performance driver. Simplified workflows solve many problems, but adding scalability significantly magnifies the impact, particularly in labs running hundreds or thousands of samples. seqWell has engineered two key simple and scalable library prep workflows based on it’s next-generation TnX transposase – the ExpressPlex workflow & seqWell directional tagmentation.
Simplicity + Scalability = Better Outcomes
- Run larger, more ambitious experiments
- Accelerate timelines across projects
- Produce better data and higher confidence
- Expand insights without expanding resources
The day-to-day impacts of seqWell’s engineered library prep workflows:
- Higher Throughput – Process more samples, faster
- Improved Data Reliability – Reduce errors and variability for higher confidence
- Faster Turnaround – Get answers when timing matters
- Higher Productivity & Lower Costs – Do more without increasing headcount or operating budget
- Broader Adoption – Lower barriers across teams and environments with no specialists required
Simplicity Starts with the Transposase
seqWell’s NGS library prep solutions focus on transposases. These enzymes can mediate a single-step reaction that both fragments and tags DNA with adapter sequences – a process frequently referred to as tagmentation. Tagmentation inherently simplifies library preparation by eliminating the multi-step processes of fragmentation, end-repair, and adapter ligation that other methods rely on.
Tn5 is the transposase most commonly used within traditional library preparation workflows. seqWell harnessed fit-for-purpose enzyme engineering to develop its proprietary TnX, next generation transposase to enhance sequencing performance, workflow robustness, and NGS data quality & uniformity.
The ExpressPlex Workflow: One-step Library Prep
Powering three library prep solutions:
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ExpressPlex 2.0
Ready-to-use, one-step library preparation kit for plasmid & amplicon sequencing.
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AgriPrep
Ready-to-use, one-step library preparation kit for high-throughput, low-pass sequencing of plant, animal, & human genomic DNA
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ExpressPlex Plus – Early Access
Inquire about our ready-to-use, one-step library preparation kit for microbial sequencing
seqWell developed the ExpressPlex Workflow for scalable simplicity, enabling scientists to process more samples, faster, with consistent performance at any throughput. It combines TnX transposase for improved performance and robustness with protocol innovations providing for a 100-minute method without sacrificing sequence coverage uniformity. Features include:
- One-step tagmentation & amplification
- Auto-normalization & early pooling
- Index & ready-reaction plates provided
- Easily automated
- Unmatched multiplexing capabilities
seqWell Directional Tagmentation
The Ease of Tagmentation, with the Performance of Ligation
seqWell’s directional tagmentation blends the workflow simplicity of tagmentation, the reduced insertion bias of TnX, & the performance benefits of ligation to power our MosaiX Library Prep Kit. This 90-minute workflow uniquely combines tagmentation with ligation to ensure all tagging events result in sequencable P5/P7 barcoded products without sacrificing simplicity.
- Combines tagmentation & ligation
- Up to 50% faster than other tagmentation & fragmentation/ligation methods
- Broad application-compatibility including those needing higher library complexity
- Flexible indexing strategy
The Takeaway
Across the growing diversity of NGS applications—from therapeutics to agriculture—the same principle holds: workflow design ultimately defines what’s possible. It’s not just simplicity that matters. Simplicity reduces friction and makes workflows easier to run, but for many labs without scalability, those same workflows can quickly become bottlenecks as throughput increases bringing progress to a halt. seqWell understands this and has harnessed transposase innovation and workflow optimization to engineer truly unique workflows and products that focus on simple scalability while providing the quality libraries you require.
seqWell™ Library Preparation Kits and Reagents
Our library prep and multiplexing kits are available to support all your short and long-read sequencing workflows. Contact us to find out which is right for you!