Tagify™ TnX Universal Adapter-loaded Transposase
Next-generation TnX™ transposase pre-loaded with Illumina-compatible universal adapters for simple, reliable NGS library preparation.
- Rapid tagmentation-based library prep
- TnX for uniform sequence coverage
- Fully QC’d for batch-to-batch consistency
- Supports a variety of applications, including ATAC-Seq
Tagify TnX Universal Adapter-loaded Transposase
Tagify TnX Universal Adapter-Loaded Transposase is ideal for simple, reliable, high-performance library preparation to support a variety of applications, such as ATAC-Seq and other NGS assays that rely on Illumina-compatible sequence adapters.
- Rapid library prep: simultaneously fragment and tag DNA via loaded transposase
- Ready-to-use: TnX pre-loaded with Illumina-compatible universal adapters
- Uniform sequence coverage: next-generation TnX transposase with increased activity, enzyme robustness and reduced insertion bias
- Trusted results: fully QC’d loaded transposase for batch-to-batch consistency
Tagify TnX P5
Adapter sequence: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’ (33 bases)
Concentration: 0.2 units* / µL
Tagify TnX P7
Adapter sequence: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’ (34 bases)
Concentration: 0.2 units* / µL
Mosaic End (ME) sequence underlined
*Unit definition: One unit is the amount of tagging reagent required to fragment 100 ng of E. coli genomic DNA to an average length of 650 – 850 bp in 5 minutes at 55°C in 1X Coding Buffer in a 50 µL total reaction volume.
Looking for a transposases loaded with a different payload?
Check out our Tagify Custom-loaded Transposase Reagents
Tagify TnX Universal Adapter Specifications
| Specs | Description |
|---|---|
| Primary Applications | NGS assays requiring Illumina-compatible sequence adapters |
| Sample Types |
|
| Kit Sizes (units*) |
|
| Transposase | TnX |
| Barcoding Strategy |
|
| Average Output Fragment Range** (measured with Agilent DNA BioAnalyzer Chip) | 650 – 850 bp |
| Sequencer Compatibility | All Illumina sequencing platforms; Use with Element Biosciences AVITI™ or other sequencing platforms is possible with conversion kits for Illumina libraries |
*1 unit of Universal adapter-loaded TnX is defined as the amount of tagging reagent required to fragment 100 ng E. coli genomic DNA to an average length of 650-850 bp in 5 minutes at 55°C in 1X Coding Buffer in a 50 μL total reaction volume. The average fragment length is determined by electrophoresis on the Agilent TapeStation.
**Because the tagged product in not a complete NGS library, sizing data and traces obtained from other methods, such as Agilent TapeStation or Femto Pulse, may vary.