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Meet purePlex™ DNA Library Prep Kit

Maximize Your Sequencing Efforts

  • 2.5-hour workflow for 96 samples, 45 min. hands-on time

  • Auto-normalization of read counts and insert size over 10-fold input range

  • Unique dual indices included

  • Significant cost and plastics savings over other transposase-based systems

SKU: 301067 | $2,015.00 USD

Fast, Easy Workflow with purePlex™

  • purePlex saves 50% total time over Nextera XT

  • 75% hands-on time savings

  • All times based on processing of 96 samples

Learn more about how purePlex technology can maximize your sequencing efforts.

Save over 50% of time by using purePlex. Check out the workflow comparison

 

Saving $$$ and The Planet Through Less Plastic

  • 50% + savings in plastics per sample

  • $7+ per plate savings

 

Complete the form to be contacted by a seqWell representative.



Benefits of purePlex:

  • Fast, flexible workflow with no requirement for full plate processing

  • Auto-normalization reduces QC burden, improves data consistency

  • Early pooling for easier sample handling

  • Reduced GC bias compared to other transposase-based methods

  • Significant cost and plastics savings

 

 

Product Highlights

Auto-normalization of insert size and read depth:

 

The insert size within a pool of samples is consistent regardless of input (panel A, left) or GC content (panel B, lower left). In contrast, Nextera XT libraries have varied fragment distributions from GC content even after normalizing the sample input.

 

Auto-normalization of Read Depth

 

Samples were normalized to inputs of 3, 5, 10, and 30 ng then underwent purePlex library prep with (+) and without () normalization reagent. Read counts for each sample are equal, regardless of input, when normalization reagent is used. In contrast, without normalization reagent, sample read count scales with input.

Specifications

Specs Description
Sample Type Amplicons, Plasmids, Genomic DNA, cDNA
DNA Input Range 5 – 50 ng
Number of Unique Index Combinations 96 (more in development)
Supported Paired Reads (Clusters)/Sample ≤ 20 million
Output Fragment Range 400 – 1,200 bp
Applications Synthetic construct sequencing (amplicons, plasmids, etc.), Low-pass whole genome sequencing, Whole small genome sequencing (<50 Mb), scRNA-seq, Metagenomics/Microbiome screening
Reactions per Kit 96

 

plexWell kit art 2

purePlex DNA Library Prep Kit Includes:

  • i7 Tagging Reagent Plate

  • i5 Tagging Reagent Plate

  • Coding Buffer (3X)

  • X Solution

  • MAGwise Paramagnetic Beads

  • Normalization Reagent

  • Library Primer Mix

Have questions? Complete the form to be contacted by a seqWell representative. We’ll be happy to assist!



Enabling Sequencing Applications with Improved Transposase-Based SolutionsUPCOMING WEBINAR

Date:  October 19, 2022
Time: 12:00pm (PDT),  3:00pm (EDT), 9:00pm (CEDT)

Learning Objectives

  • Understand the utility of Tn5 transposase and tagmentation across a range of NGS applications
  • Recognize how transposase-based library prep methods permit significant improvements in sequencing workflows without compromising data quality
  • Identify opportunities for incorporating custom Tn5 transposase solutions that allow new innovations in your lab

Speakers

Joseph C. Mellor, Ph.D.
Co-founder and Chief Scientific Officer, seqWell

Jessica M. Smith, Ph.D.
Director of Bioinformatics, seqWell

Curtis Knox
Director of Product Management, seqWell