Auto-Normalization of Insert Size and Read Depth:
Samples were normalized to inputs of 3, 5, 10, and 30 ng then underwent purePlex library prep with (+) and without (–) normalization reagent. Read counts for each sample are equal, regardless of input, when normalization reagent is used. In contrast, without normalization reagent, sample read count scales with input.
|Sample Type||Amplicons, Plasmids, Genomic DNA, cDNA|
|DNA Input Range||5 – 50 ng|
|Number of Unique Index Combinations||96 (more in development)|
|Supported Paired Reads (Clusters)/Sample||≤ 20 million|
|Output Fragment Range||400 – 1,200 bp|
|Applications||Synthetic construct sequencing (amplicons, plasmids, etc.), Low-pass whole genome sequencing, Whole small genome sequencing (<50 Mb), scRNA-seq, Metagenomics/Microbiome screening|
|Sequencer Compatibility||All Illumina SequencerspurePlex DNA Library Prep Kit Includes:|
|Reactions per Kit||96|
|Reactions per Batch||All Illumina SequencerspurePlex DNA Library Prep Kit Includes:|
Frank A. Middleton, Ph.D.
Professor Director, SUNY Molecular Analysis CoreState University of New York (SUNY) Upstate Medical University
Joseph C. Mellor, Ph.D.
Co-founder and Chief Scientific Officer of seqWell Inc.