Learn how the purePlex DNA library prep system offers speed, performance, and auto-normalization with unique dual indexes with Maura Costello, Team Leader, R&D, at seqWell.
Auto-Normalization of Insert Size and Read Depth:
The insert size within a pool of samples is consistent regardless of input (panel A, left) or GC content (panel B, lower left). In contrast, Nextera XT libraries have varied fragment distributions from GC content even after normalizing the sample input.
Samples were normalized to inputs of 3, 5, 10, and 30 ng then underwent purePlex library prep with (+) and without (–) normalization reagent. Read counts for each sample are equal, regardless of input, when normalization reagent is used. In contrast, without normalization reagent, sample read count scales with input.
|Sample Type||Amplicons, Plasmids, Genomic DNA, cDNA|
|DNA Input Range||5 – 50 ng|
|Number of Unique Index Combinations||96 (more in development)|
|Supported Paired Reads (Clusters)/Sample||≤ 20 million|
|Output Fragment Range||400 – 1,200 bp|
|Applications||Synthetic construct sequencing (amplicons, plasmids, etc.), Low-pass whole genome sequencing, Whole small genome sequencing (<50 Mb), scRNA-seq, Metagenomics/Microbiome screening|
|Reactions per Kit||96|