Description:

  • Same enhanced plexWell workflow engineered for low-pass whole genome library prep and sequencing
  • Kit contains major reagents necessary to prepare libraries including Magwise™ paramagnetic beads (DNA polymerase not included)
  • Efficient library prep for more than one 96-well plate of samples
  • Normalizes input DNA over wide input range of 3-30 ng
  • Volume-based pricing (save 30-50% on total lab costs)

Recommended Application:

  • Low-depth whole genome/GBS coupled with an imputation and analysis software

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

Specifications:

    • Time-to-prepare a multiplexed library from 96 samples:
      • Hands-on time:  1 hr
      • Total elapsed time:  3 hrs
    • Recommended input: 3-30 ng of genomic DNA per sample (n=96)
    • Expected Results:
      • Library Yield: 750 – 1500 fmoles of purified multiplexed library
      • Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
      • Substantial normalization: Typically <2.5-fold range (min to max) in read count across all 96 samples
    • Compatibility with Illumina sequencing systems:
      • Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms

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