The simplicity of the ExpressPlex workflow makes it uniquely well-suited for manual, automated, and ultra high throughput library prep from plasmids and PCR products.
– Lacy Simons, Acting Lab Manager of the Hultquist Lab and
Lab Director of the Center for Pathogen Genomics and Microbial Evolution,
The game-changing capabilities enabled by ExpressPlex were highlighted in a webinar collaboration with the synthetic biology team from Octant Bio, a drug discovery company located in Emeryville, CA. Octant’s Henry Chan, PhD (Synthetic Biology Lead) and Bryan Jiang (Research Associate) discussed the application of ExpressPlex to OCTOPUS, the high-throughput plasmid sequencing platform that powers Octant’s therapeutic discovery platform. Watch the video to discover how ExpressPlex has dramatically improved OCTOPUS and accelerated their overall discovery process.
ExpressPlex allows you to spend your time on data and results, not pipetting. There are solutions for low-, medium-, and high-throughput labs. For ultra-high throughput users, we now offer 384-well versions that will enable you to multiplex up to 6,144 samples in a single run.
In this 5-minute video, we walk you through the single-step workflow to demonstrate the speed and simplicity of the auto-normalizing ExpressPlex technology. The ExpressPlex Library Prep Kit is an ideal solution for high-throughput multiplexed sample preparation for plasmid and amplicon sequencing.
ExpressPlex demonstrates significantly higher levels of normalization compared to competitors, enabling a simplified workflow where individual normalization is no longer required to achieve more consistent read-depths across samples.
Libraries were prepared from three different reference plasmids of varying sizes across a 10-fold input range at full reaction volume for ExpressPlex (16 µL) and Nextera XT (50 µL), as well as at ¼ miniaturized reaction volume for Nextera XT.
For each method, inputs ranged from 4-40ng for ExpressPlex (standard input is 16ng) and 0.4 – 4ng for Nextera XT (standard input is 1ng). Inputs were scaled appropriately for 1/4 reactions.
ExpressPlex generates highly consistent library insert sizes over a broad range of DNA inputs.
Given the robust read balance and lower number of reads required to generate correct assemblies, ExpressPlex users can multiplex higher numbers of samples on a single Illumina run or utilize a lower capacity flow cell, thereby reducing the cost of sequencing per plasmid.
ExpressPlex’s workflow and 10bp barcodes typically provide >99% on-target alignment rates.
Thanks to the highly efficient ExpressPlex workflow, almost 80% fewer pipette tips and plastics are used to prepare your libraries.
Calculations are based on 96 samples prepared, comparing ExpressPlex to Nextera XT.
|Primary Applications*||Plasmid and amplicon sequencing|
|Sample Types*||Plasmids, amplicons > 350bp|
|Reactions per Kit||96 reactions
384 (4 different sets available)
384-well version now available in early access
|DNA Input Recommended||8 – 40ng|
|Total Library Prep Time (hands-on time)||90 min
(30 min hands-on)
|Indexing Method||Combinatorial Dual Indexing|
|Number of Unique Index Combinations||1,536
6,144 now available in early access
|Supported Paired Reads (Clusters) per Sample||< 4 million (validated to date)|
|Output Fragment Range**||400 – 1,200bp|
|Number of PCR Cycles||12 cycles for plasmids
15 cycles for amplicons
|Sequencer Compatibility||All Illumina sequencing platforms;
Use with Element Biosciences AVITI™, Complete Genomics G99, or other sequencing platforms is possible with conversion kits for Illumina libraries
*Other sample types are compatible. Contact seqWell’s application group for guidance.
**Fragment size will depend on magnetic bead cleanup ratios used.