Plasmids are ubiquitously used in recombinant DNA technology for the cloning, engineering, transfer, and expression of DNA fragments or genes. Broad-based access to cost-effective, massively parallel, next-generation sequencing (NGS), and advances in synthetic biology have revolutionized the scale on which plasmids can be constructed, screened, and manipulated for wide-ranging applications, including biomarker discovery, antibody engineering, genome editing, and gene therapy.
NGS library preparation throughput, performance, and cost remain bottlenecks in high-throughput plasmid sequencing. In this study, we demonstrate the utility of plexWell Library Preparation Kits for high-quality, high-throughput plasmid sequencing on benchtop sequencers.
Joseph C. Mellor, Ph.D.
Co-founder and Chief Scientific Officer, seqWell
Jessica M. Smith, Ph.D.
Director of Bioinformatics, seqWell
Director of Product Management, seqWell