The plexWellTM Library Prep kit offers the most efficient and optimized workflow for your DNA sequencing applications.

Product Highlights:

  • Wide range of applications
  • Auto-normalization, no quant necessary
  • Fast prep for 96 x 96 plates
  • Fewer hands on steps
  • Wide range of DNA input
  • High quality, reproducible data
  • Automation ready

Save Money and Labor

plexWell Library Prep Kits typically cost 30 – 50% of conventional library prep on a per sample basis (volume-dependent), but additional savings and throughput are realized in a few different ways:

  • Reduced Sample QC cost – No need to quantify any sample on the plate or spend time normalizing all samples to a fixed input
  • Reduced Library QC cost – pooled library prep removes 1 sample to 1 library QC necessity, 1 QC per 96 samples is enabled
  • Labor/Time savings due to ease of multiplexing (ability to multiplex up to 9216 (96 x 96) samples in a sequencing run) 

High-throughput Workflow

plexWell’s optimized workflow is enabled by a unique “pooled library prep” step, in which a relatively large number of samples are subjected to a DNA-barcoding step that uniquely labels DNA from each well of a 96-well plate, and then samples are pooled together to create a single library containing random nested fragments carrying constituent barcodes.

Normalization Performance Example: >90% of input samples at 0.5% or higher output representation; >80% of input samples within 4-fold output range; 1.5 log normalization effect within support input range (100-fold input range reduced to 2.5 output range)
E. coli library preparation with plexwell gives consistently longer median inserts , maximizing the per read usable data. Insert sizes are consistent over a range of inputs.

Product Specifications

Pricing and Kit Configurations

plexWell-96 Library Prep Kit

  • plexWell NGS multiplexed library generation kit for Illumina®
  • Kit contents include all reagents necessary to prepare Illumina-compatible NGS libraries from 1 x 96-well plates of input DNA
  • 96 unique barcode combinations

plexWell-96 List Price = $1920 ($20 per sample)

plexWell-384 Library Prep Kit

  • plexWell NGS multiplexed library generation kit for Illumina®
  • Kit contents include all reagents necessary to prepare Illumina-compatible NGS libraries from 4 x 96-well plates of input DNA
  • 384 unique barcode combinations

plexWell-384 List Price = $5760 ($15 per sample)

Components of plexWell-96 and plexWell-384 Kits:

-20°C Box Components:

  • Well-specific Barcoding Plates (WB Reaction Plates), preloaded, color-coded.
  • Plate-specific Barcode Reagent (PB), color-coded to match WB plates
  • Library Primer Mix

Room Temp Box Components:

  • Coding Buffer
  • X Solution
  • MAGwiseTM Paramagnetic Beads
  • Empty 2 ml tubes, color-coded to match WB plates

40% introductory discount on plexWellTM kits, fill out form to order kit:

What plexWell kit configuration are interested in?