Resolution at Scale:
plexWell Rapid Single Cell for scRNA sequencing

Overview

New methods to interrogate the transcriptional behavior of individual cells have been created, which has driven exponential growth in our ability to study and understand biology at higher resolution. Amid this growth, different molecular tools are clearly needed to understand and measure cells at different scales and by different modalities. In single-cell mRNA (scRNA) sequencing, an emerging consensus is that sequencing and counting transcript ends (e.g., digital gene expression counting) in high numbers of cells can often be powerfully complemented by deeper sequencing of whole transcripts (e.g., full-length cDNA) from a smaller number of cells. These latter, more focused studies are often addressed with plate-based cell workflows such as Smart-seq2 or related methods that work from plated single cells in sorted or microwell format.

The information gain from plate-based scRNA sequencing methods can be quite significant ­­– more transcripts detected per cell, with full-length transcript information – but the conventional challenge with these workflows is often the lack of ready-to-use, cost-effective and scalable solution to support them. To solve and address this challenge, seqWell has developed a new product ­for capturing and sequencing single-cell transcripts from hundreds to thousands of cells in a simple, scalable plate-based format. The plexWell Rapid Single Cell Kit couples sensitive, easy-to-use cDNA synthesis chemistry with the unique multiplexed normalizing plexWell library preparation technology, to allow researchers to product auto-normalized libraries in a single day for hundreds to thousands of cells and profile single-cell whole transcripts from a wide range of cell types.

In this presentation, we demonstrate how plexWell Rapid Single Cell offers significant improvements in workflow and performance versus other plate-based workflows, and offers a compelling ready-to-use tool for focused single-cell mRNA sequencing studies that enhances and complements other single-cell assays. We explore the comparative performance and benefits versus existing technologies on peripheral blood mononuclear cells (PBMCs), and discuss how plate-based workflows offer a powerful complement to 10X Chromium for building rich datasets from more comprehensive single-cell study designs.

This webinar will be presented by Simone Picelli from the Institute Ophthalmology Basel with a short introduction by Joe Mellor, CEO of seqWell.