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Overview

New methods to interrogate the transcriptional behavior of individual cells have been created, which has driven exponential growth in our ability to study and understand biology at higher resolution. Amid this growth, different molecular tools are clearly needed to understand and measure cells at different scales and by different modalities. In single-cell mRNA (scRNA) sequencing, an emerging consensus is that sequencing and counting transcript ends (e.g., digital gene expression counting) in high numbers of cells can often be powerfully complemented by deeper sequencing of whole transcripts (e.g., full-length cDNA) from a smaller number of cells. These latter, more focused studies are often addressed with plate-based cell workflows such as Smart-seq2 or related methods that work from plated single cells in sorted or microwell format.

The information gain from plate-based scRNA sequencing methods can be quite significant ­­– more transcripts detected per cell, with full-length transcript information – but the conventional challenge with these workflows is often the lack of ready-to-use, cost-effective and scalable solution to support them. To solve and address this challenge, seqWell has developed a new product ­for capturing and sequencing single-cell transcripts from hundreds to thousands of cells in a simple, scalable plate-based format. The plexWell Rapid Single Cell Kit couples sensitive, easy-to-use cDNA synthesis chemistry with the unique multiplexed normalizing plexWell library preparation technology, to allow researchers to product auto-normalized libraries in a single day for hundreds to thousands of cells and profile single-cell whole transcripts from a wide range of cell types.

In this presentation, we demonstrate how plexWell Rapid Single Cell offers significant improvements in workflow and performance versus other plate-based workflows, and offers a compelling ready-to-use tool for focused single-cell mRNA sequencing studies that enhances and complements other single-cell assays. We explore the comparative performance and benefits versus existing technologies on peripheral blood mononuclear cells (PBMCs), and discuss how plate-based workflows offer a powerful complement to 10X Chromium for building rich datasets from more comprehensive single-cell study designs.

This webinar will be presented by Simone Picelli from the Institute Ophthalmology Basel with a short introduction by Joe Mellor, CEO of seqWell.



Simone Picelli

Simone Picelli PhD
Team Leader, Single Cell Genomics Platform
Institute Ophthalmology Basel
Dr. Picelli currently works as Team Leader in the Single Cell Genomics Platform at the Institute Ophthalmology Basel (IOB) in Basel, Switzerland. He obtained his PhD in Biotechnology from the University of Padova (Italy) in 2006 before moving to Stockholm for a first postdoc at the Karolinska Institute. After a short spell at the German Cancer research Centre (DKFZ) in Heidelberg, he returned to Stockholm in 2012 for a second postdoc at the Ludwig Institute for Cancer Research (LICR) in the group of Rickard Sandberg. There he developed the Smart-seq2 and an in-house version of Tn5 Transposase for single-cell library preparation. In 2015 he joined the Eukaryotic Single Cell Genomics Facility that Rickard Sandberg and Sten Linnarsson opened at the Science for Life Laboratory (also in Stockholm).
You can find Simone on LinkedIn.

Joseph Picture

Joseph Mellor PhD
Co-founder and CEO
seqWell

Dr. Mellor is co-founder and CEO at seqWell, Inc., a genomics solutions company started in 2014 with the goal of supporting transformative genomic applications with simple, scalable products and solutions. Joe is a computational and molecular biologist with previous research positions at Harvard Medical School and the University of Toronto. He is an inventor of several technologies in the area of DNA library preparation and sequencing. He obtained a PhD in Bioinformatics from Boston University, and a BS in Chemistry from the University of Chicago. You can find Joe on LinkedIn.

Enabling Sequencing Applications with Improved Transposase-Based SolutionsUPCOMING WEBINAR

Date:  October 19, 2022
Time: 12:00pm (PDT),  3:00pm (EDT), 9:00pm (CEDT)

Learning Objectives

  • Understand the utility of Tn5 transposase and tagmentation across a range of NGS applications
  • Recognize how transposase-based library prep methods permit significant improvements in sequencing workflows without compromising data quality
  • Identify opportunities for incorporating custom Tn5 transposase solutions that allow new innovations in your lab

Speakers

Joseph C. Mellor, Ph.D.
Co-founder and Chief Scientific Officer, seqWell

Jessica M. Smith, Ph.D.
Director of Bioinformatics, seqWell

Curtis Knox
Director of Product Management, seqWell