Pre-plated Reagents, Simple Workflow: Less Resources Required

ExpressPlex HT vs. Illumina DNA Prep: Resource Comparison

Massive Multiplexing + Normalization = Less Effort to Run Your Samples

The ExpressPlex HT kit combines the largest ready-made CDI barcode set with a built-in read depth normalization feature, enabling greater capacity and consistent read depths across all samples.

The ExpressPlex HT kit was used to prepare fixed input (8ng) libraries for 6,144 total pUC19 samples across 16 separate 384-well plates. All samples were pooled into a single run on a NextSeq 2000 sequencer (P1 300 cycle @ 2×150). Library preparation was performed on either a Tecan Evo or SPT Apricot. As shown above, even when multiplexing 6,144 samples, ExpressPlex HT provides consistent read depths sufficient for easy assembly of plasmid samples.

Skip plasmid prep and get even faster results

ExpressPlex HT is compatible with plasmid DNA prepared by rolling circle amplification (RCA) or direct colony PCR.

384 plasmids, ranging in size from 4 to 8kb, were picked from agar plates and amplified directly from colony lysates using RCA (EquiPhi29™, Thermo Fisher), or colony PCR (KOD One™, Toyobo). 384-plex libraries were prepared from unpurified RCA and colony PCR reactions using ExpressPlex HT kits on the Apricot S3 Liquid Handler (SPT Labtech). Sequencing was conducted on the MiSeq™ sequencing platform on a Micro v2 kit (384 samples per run).