Tagify™ i5 UMI Adapter-loaded Transposase reagents are for tagmentation-based NGS library preparation for simple, scalable, and reliable unique molecular identifier (UMI) addition. Accelerate your variant calling efforts with peace of mind.
GUIDE-seq2 , the second generation of GUIDE-seq, delivers a more sensitive, reproducible and scalable assay by integrating tagmentation into the library preparation workflow, while retaining the assay’s proven biology. Tagify i5 UMI is a commercial source of fully QC-ed, ready-to-use loaded transposase that unleashes the power of GUIDE-seq2!
In this webinar, Dr. Giannoukos highlighted how her team combined a custom Tagify reagent with their UDiTaS method to measure large deletions and inversions at the CEP290 editing site.
She also incorporated data demonstrating reproducibility of different Tagify batches. This webinar was transcribed into a blog if you prefer to read along.
Specs | Description |
---|---|
Primary Applications | CRISPR or Gene Editing QC and Verification |
Sample Types | Genomic DNA |
Reactions per Kit |
|
Standard dispense volume per well | 3μl or 4μl in a 96-well microtiter plate |
Transposase | Hyperactive Tn5 |
DNA Input Recommended | 50ng |
Number of unique barcodes | 24 i5 indexes with unique molecular identifiers(UMI). Inquire for additional i5 indexes at sales@seqwell.com |
Barcoding Strategy |
|
Standard Output Fragment Range** (measured with Agilent DNA BioAnalyzer Chip) | 800 – 2000 bp (using region mode with a range of 200 – 7500 bp) |
Sequencer Compatibility | All Illumina sequencing platforms; Use with Element Biosciences AVITI™ or other sequencing platforms is possible with conversion kits for Illumina libraries |
**Because the tagged product in not a complete NGS library, sizing data and traces obtained from other methods, such as Agilent TapeStation or Femto Pulse, may vary.
Applications* – commercial use may require a license from a third party.
1. UDiTaS Method: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861650/
3. RGEN-Seq Method: https://pubmed.ncbi.nlm.nih.gov/34880355/