Description:

  • Kit contains major reagents necessary to prepare libraries including Magwise™ paramagnetic beads (DNA polymerase not included)
  • Efficient library prep for one 96-well plate inputs
  • Normalizes input DNA over wide input range of 3-30 ng
  • Volume-based pricing (save 30-50% on total lab costs)

Recommended Applications:

  • Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc)
  • Microbiome screening
  • Microbial whole genome sequencing
  • scRNA-seq
  • Low-depth whole genome/GBS

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

Specifications:

  • Time-to-prepare a multiplexed library from 96 samples:
    • Hands-on time:  1 hr
    • Total elapsed time:  3 hrs
  • Recommended input: 3-30 ng of genomic DNA per sample (n=96)
  • Expected Results:
    • Library Yield: 750 – 1500 fmoles of purified multiplexed library
    • Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
    • Substantial normalization: Typically <2.5-fold range (min to max) in read count across all 96 samples
  • Compatibility with Illumina sequencing systems:
    • Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms