ExpressPlex™ Library Prep Kit
ExpressPlex, the original one-step prep, is designed as the ultimate in high-throughput library prep for plasmid and amplicon screening, with a streamlined workflow and unmatched multiplexing capabilities.
- 90-min workflow with 30-min or less hands-on
- One-step library prep – fragmentation, barcoding, and amplification in a single step
- Up to 6,144 barcodes available (combinatorial dual indices)
- Available in 96- and 384-microtiter plate configurations or as bulk reagents
Looking to take your plasmid and amplicon sequencing to the next level?
Check out ExpressPlex 2.0
The workflow simplicity of the original ExpressPlex PLUS the improved performance of TnX next generation transposase!
Recorded Webinar | Octant Bio
The game-changing capabilities enabled by ExpressPlex were highlighted in a webinar collaboration with the synthetic biology team from Octant Bio, a drug discovery company located in Emeryville, CA. Octant’s Henry Chan, PhD (Synthetic Biology Lead) and Bryan Jiang (Research Associate) discussed the application of ExpressPlex to OCTOPUS, the high-throughput plasmid sequencing platform that powers Octant’s therapeutic discovery platform. Watch the video to discover how ExpressPlex has dramatically improved OCTOPUS and accelerated their overall discovery process.
“ExpressPlex is literally faster than Sanger. This changes everything for us. Basically, taking a two-day process to one day dramatically shortens time to data.”
– Henry Chan, Ph.D., Synthetic Biology Lead, Octant Bio
Benefits of using ExpressPlex
ExpressPlex allows you to spend your time on data and results, not pipetting. This one-step library prep provides a solution for low-, medium-, and high-throughput labs.
- Rapid: Go from extracted samples to libraries on a sequencer in < 1/2 a day
- Massive multiplexing: supports ultra-high throughput screening
- Scalable: easily automated of your platform-of-choice
- Reduce labor while increasing efficiency
- Everything supplied in index & ready reaction mix plates: no complex supply chain management of barcodes and primers
- Improve lab sustainability: significant reduction in tips/plastics usage
ExpressPlex Library Prep Kit: Your ticket to speed, throughput, efficiency
The simplicity of the ExpressPlex workflow makes it uniquely well-suited for manual, automated, and ultra high throughput library prep from plasmids and PCR products.
ExpressPlex 96- Well Workflow
ExpressPlex HT 384-Well Workflow
Help Save the Environment with Less Plastics
Thanks to the highly efficient ExpressPlex workflow, almost 80% fewer pipette tips and plastics are used to prepare your libraries.
Calculations are based on 96 samples prepared, comparing ExpressPlex to Nextera XT.
Train any lab tech, minimize chance for error with ExpressPlex
“ExpressPlex eliminates so many bead cleanup steps, which is usually where people struggle to get recovery from or experience uncertainty. ExpressPlex is so much better for everybody involved and makes it way easier for my new staff to catch up and feel comfortable doing library prep.”
– Lacy Simons, Acting Lab Manager of the Hultquist Lab and
Lab Director of the Center for Pathogen Genomics and Microbial Evolution,
Northwestern University
Product Highlights
Auto-Normalization of Read Depth
ExpressPlex demonstrates significantly higher levels of normalization compared to competitors, enabling a simplified workflow where individual normalization is no longer required to achieve more consistent read-depths across samples.
Libraries were prepared from three different reference plasmids of varying sizes across a 10-fold input range at full reaction volume for ExpressPlex (16 µL) and Nextera XT (50 µL), as well as at ¼ miniaturized reaction volume for Nextera XT.
For each method, inputs ranged from 4-40ng for ExpressPlex (standard input is 16ng) and 0.4 – 4ng for Nextera XT (standard input is 1ng). Inputs were scaled appropriately for 1/4 reactions.
Auto-Normalization of Insert Size
ExpressPlex generates highly consistent library insert sizes over a broad range of DNA inputs.
Libraries from pUC19 plasmid DNA were simultaneously prepared at 3 different input amounts (n=8 each input amount). ExpressPlex demonstrated greater consistency in average insert size across a 10-fold range. In each case, the standard input for both kits (16ng for ExpressPlex, 1ng for Nextera XT) was also included as a mid-range data point.
Success Rates of Plasmid Assembly
Libraries were prepared as described above, sequenced (MiSeq Nano; 2 x150) and de novo assembled using seqWell’s SNAP plasmid assembly pipeline. The sequencing run was downsampled to either 1,000 or 500 average read pairs per sample to identify error-free, circular plasmid assemblies. Even at only 500 average read pairs all ExpressPlex samples generated correct circularized assemblies. At the same number of reads, however, Nextera XT (full and ¼ reactions) failed 8-10% of assembly attempts.
Given the robust read balance and lower number of reads required to generate correct assemblies, ExpressPlex users can multiplex higher numbers of samples on a single Illumina run or utilize a lower capacity flow cell, thereby reducing the cost of sequencing per plasmid.
Confident Amplicon Sequencing
ExpressPlex’s workflow and 10bp barcodes typically provide >99% on-target alignment rates.
20 uniquely mapping amplicons were generated from lambda DNA. The amplicons were used combinatorial as input into the ExpressPlex library prep such that each sample is comprised of 2 lambda amplicons and each well contains a unique amplicon combination. Sample input was maintained at 16 ng and 15 cycles of PCR were used during the library preparation. Following sequencing, demultiplexed reads were mapped to the lambda genome and marked as on-target (expected amplicon combination) or off-target for each well. All wells have an on-target rate >99% (above figure) with an average on-target rate of 99.XX%. Amplicon sequencing with EP enables efficient preparation of up to 1536 amplicons multiplexed on a single lane, enabling high-throughput applications without fear of index-hopping, or PCR chimeras.
Are you in need of bulk-dispensed product for your miniaturized reactions?
ExpressPlex Specifications
| Specs | Description |
|---|---|
| Primary Applications | Plasmid and amplicon sequencing |
| Sample Types* | Purified plasmids, RCA-amplified DNA, colony PCR amplicons, amplicons > 350bp |
| Reactions per Kit | 96 or 1536 reactions (custom dispense options available) |
| DNA Input Recommended | 8 – 40ng |
| Total Library Prep Time (hands-on time) | 90 minutes
(30 minutes hands-on) |
| Indexing Method | Combinatorial Dual Indexing |
| Number of Unique Index Combinations | Up to 6144 |
| Output Fragment Range** | 400 – 1,200bp |
| Number of PCR Cycles | 12 cycles for plasmids 15 cycles for amplicons |
| Sequencer Compatibility | All Illumina sequencing platforms; Use with Element Biosciences AVITI™, Complete Genomics G99, or other sequencing platforms is possible with conversion kits for Illumina libraries |
*Other sample types are compatible. Contact seqWell’s application group for guidance.
**Fragment size will depend on magnetic bead cleanup ratios used.
ExpressPlex Library Prep Kit Includes:
- Indexing Reaction Plate(s)
- Ready Reaction Mix Plate(s)
- MAGwise Paramagnetic Beads
- Users do not need to supply polymerase, primers, or barcodes. These are already pre-mixed into the reagents supplied.