This poster presentation highlights how the plexWell LP 384 library prep kit provides a streamlined, scalable, and reproducible solution for routine crop genotyping across multiple plant species with different genome sizes.
This poster demonstrates the ability to generate highly multiplexed pools of lcWGS libraries from Cannabis sativa using an automation workflow on a Sciclone G3 NGSx Workstation liquid handling workstation from PerkinElmer, Inc. and seqWell’s purePlex™ library preparation technology for sequencing on an Illumina NovaSeq instrument.
This poster showcases seqWell’s transformational library prep technology that enables barcoding and amplification of 8 – 384 dual-indexed libraries in 90 minutes in a “one-pot” reaction, while simultaneously auto-normalizing library read count and insert size.
The purePlex™ DNA Library Prep Kit supports truly multiplexed and highly scalable construction of library pools for low-pass WGS. Together with the Element AVITI™ system, the NGS technology offers an accessible and robust alternative to microarrays for genotyping.
This application note demonstrates that the purePlex DNA Library Prep Kit paired with the AVITI System improves the performance of mid-throughput, low-pass WGS at a competitive price point.
Early access users of ExpressPlex™ agree that the library prep is so easy and quick. With the significantly shortened hands-on time, some enthusiastically reported they were able to perform prep the same day as receiving the kit!
Conventional library preparation methods demand multiple incubations interrupted by cumbersome pipetting steps. A transformational new library prep technology from seqWell enables barcoding and amplification of 8-384 dual-indexed libraries in 90 minutes in 16 µl “one-pot” reactions, while simultaneously auto-normalizing library read-count and insert size.
The simplicity of the ExpressPlex workflow makes it uniquely well-suited for manual, automated and ultra high throughput (UHT) library prep from plasmids and PCR products.