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End-to-end library preparation for scalable, cost-effective single-cell transcriptome analysis

Sensitive and robust profiling of transcripts at single-cell resolution

 

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Maximize Your Library Prep Efforts

Single-day protocol, scalable to your project needs

  • Sorted cells to sequencing-read full-length cDNA libraries in one day.

  • Scalable, plate-based workflow.

  • Auto-normalization reduces sample QC.

  • Sensitive and robust transcript identification and expression analysis.

  • More information from every cell.

  • No specialized equipment required.

Prep Kit Tray

Features and benefits

Streamlined, fully integrated workflow

  • Compatible with sorted cells or low RNA inputs (2pg – 10 ng) arrayed in plates or strip tubes

  • Novel improvements to Smart-seq2 method

  • Combined cDNA synthesis and amplification reduces hands-on and overall workflow time

  • Unique features of plexWell library prep eliminates QC and dilution of every individual cDNA sample

  • Yields normalized, amplified libraries

  • Truly multiplexed library prep is highly scalable and reduces library prep and sequencing cost

     

Learn more about how plexWell technology can maximize your sequencing efforts.

A truly integrated workflow: Save over 40% of time by using Rapid Single Cell

 

Multiplexing uniformity

plexWell auto-normalization ensures a highly even read distribution across single
cells.

  • Libraries were prepared from individual HEK293 cells and sequenced on a NextSeq® 500 instrument. All 96 cDNA samples were quantified and normalized for the Smart-seq2 workflow. For the plexWell workflow, cDNAs were diluted, by applying a global dilution factor derived from QC data for only 24 of the samples.

 

Library number vs. Proportion of reads

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Transcript sensitivity:

plexWell Rapid Single Cell RNA Library Prep Kits enable high-sensitivity gene expression analysis and highly uniform transcript coverage.

  • Performance with libraries prepared from 2 pg or 10 pg of total HEK293 RNA or single cells, using the plexWell Rapid Single Cell RNA-Seq Library Prep Kit, SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio), or the Smart-seq2 method (Picelli S. (2019).

 

 

Application highlight: High-performance scRNA-Seq of PBMCs sensitive and efficient full-length transcriptome profiling of challenging samples

 

Single cell transcriptome profiling is now routinely employed in immunological studies, to better understand heterogeneity in cell populations with overlapping phenotypic markers; cell development and differentiation; hematopoietic pathways and regulatory networks that predict immune function. Single cell RNA-Seq of peripheral blood mononuclear cells (PBMCs) is particularly challenging because of ultra-low cellular RNA content (estimated at ≤1 pg per cell). In addition to a more streamlined workflow, the plexWellTM Rapid Single Cell RNA Library Prep Kit offers improved sensitivity (more genes detected; irrespective of sequencing depth); higher efficiency (less reads mapping to undesired content) and/or more uniform full-length transcript coverage as compared to homebrewed and commercially available reagents and protocols.

 

Product Highlights

Highly reproducible performance ensures reliable results

High-quality performance with challenging samples, such as PBMCs.

Features and benefits:

  • End-to-end library preparation for scalable, cost-effective single-cell transcriptome analysis in a single day

  • High-efficiency workflow delivers full-length transcript coverage with highly sensitive transcript and gene detection

  • Novel auto-normalization reduces QC burden by 75%

  • Barcoding strategy enables multiplexing of up to 1,152 single cells for sequencing

Resolution at Scale: plexWell Rapid Single cell for scRNA Sequencing

Advantages of plate-based, full-length transcriptome sequencing

  • Complements 3’ and 5’-only droplet-based methods by expanding on cell subpopulations of interest

  • Allows for more in-depth analyses, including allele-specific expression profiling, isoform detection, and SNP and variant profiling across full-length transcripts

  • Detects more transcripts per cell, including those of lower abundance

  • Utilizes FACS instruments for cell sorting and achieves higher cell capture efficiency

  • Requires far fewer cells for successful analysis

  • Enables long-term cell storage post-sorting

Have questions? Complete the form to be contacted by a seqWell representative. We’ll be happy to assist!