Description:

  • Library prep for 24 gDNA samples for sequencing on the NovaSeq 6000
  • 24-plex for higher-complexity human/plant/animal gDNA library prep
  • Uniform >400bp insert sizing and better read balance across samples
  • Kit contains all major reagents necessary to prepare Illumina-compatible NGS libraries including MagwiseTM paramagnetic beads (DNA polymerase not included)
  • Volume-based pricing

Recommended Application:

  • High-complexity genome sequencing
    • Human
    • Animal
    • Plant

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

Specifications:

  • Time-to-prepare a multiplexed library from 24 samples:
    • Hands-on time: 1 hr
    • Total elapsed time: 3 hrs
  • Input: 200 ng of genomic DNA per sample (n=24)
  • Expected Results:
    • Library Yield: 750 – 1500 fmoles of purified multiplexed library
    • Library Diversity: <6 % dupe rate when human genomic DNA is sequenced to 30X depth
    • Substantial normalization: Typically <1.5-fold range (min to max) in read count across all 24 sample
  • Compatibility with Illumina sequencing systems:
    • Optimized for sequencing performance on the NovaSeq 6000 in cooperation with Illumina
    • Also compatible with Illumina MiSeq®, NextSeq®, HiSeq® instrument platforms