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Description:

  • Library prep for 24 gDNA samples for sequencing on the NovaSeq 6000
  • 24-plex for higher-complexity human/plant/animal gDNA library prep
  • Uniform >500bp insert sizing and better read balance across samples
  • Kit contains all major reagents necessary to prepare Illumina-compatible NGS libraries including MagwiseTM paramagnetic beads (DNA polymerase not included)

Recommended Application:

  • High-complexity genome sequencing
    • Human
    • Animal
    • Plant

plexWell™ WGS24 Library Prep Workflow:

plexWell™ WGS24 Library Prep Chemistry:

Specifications:

  • Time-to-prepare a multiplexed library from 24 samples:
    • Hands-on time: 1 hr
    • Total elapsed time: 3 hrs
  • DNA Input: 200 ng of genomic DNA per sample (n=24)
  • Expected Results:
    • Library Yield: 750 – 1500 fmoles of purified multiplexed library
    • Library Diversity: <6 % dupe rate when human genomic DNA is sequenced to 30X depth
    • Substantial normalization: Typically <1.5-fold range (min to max) in read count across all 24 sample
  • Compatibility with Illumina sequencing systems:
    • Optimized for sequencing performance on the NovaSeq 6000 in cooperation with Illumina
    • Also compatible with Illumina MiSeq®, NextSeq®, HiSeq® instrument platforms
Caught in the Act: Tracking the Emergence and Divergence of SARS-CoV-2 through Statewide Testing and SequencingUPCOMING WEBINAR

Date:  March 23, 2022
Time: 11:00am (PDT),  2:00pm (EDT), 8:00pm (CEDT)

Learning Objectives

  • Understand the utility of full-genome viral sequence analysis for public health
  • Recognize how viral load impacts the ability to detect and sequence intact SARS-CoV-2
  • Identify the similarities and differences in host responses to SARS-CoV-2 depending on strain and immunologic history

Speakers

Frank A. Middleton, Ph.D.
Professor Director, SUNY Molecular Analysis CoreState University of New York (SUNY) Upstate Medical University

Joseph C. Mellor, Ph.D.
Co-founder and Chief Scientific Officer of seqWell Inc.