As of March 31, 2023, the manufacture and sale of the plexWell WGS 24 kit has been discontinued. Please contact us for early access to future products designed for whole genome sequencing or hybrid capture applications.
Library prep for 24 gDNA samples for sequencing on the NovaSeq 6000
24-plex for higher-complexity human/plant/animal gDNA library prep
Uniform >500bp insert sizing and better read balance across samples
Kit contains all major reagents necessary to prepare Illumina-compatible NGS libraries including MagwiseTM paramagnetic beads (DNA polymerase not included)
Recommended Application:
High-complexity genome sequencing
Human
Animal
Plant
plexWell™ WGS24 Library Prep Workflow:
plexWell™ WGS24 Library Prep Chemistry:
Specifications:
Time-to-prepare a multiplexed library from 24 samples:
Hands-on time: 1 hr
Total elapsed time: 3 hrs
DNA Input: 200 ng of genomic DNA per sample (n=24)
Expected Results:
Library Yield: 750 – 1500 fmoles of purified multiplexed library
Library Diversity: <6 % dupe rate when human genomic DNA is sequenced to 30X depth
Substantial normalization: Typically <1.5-fold range (min to max) in read count across all 24 sample
Compatibility with Illumina sequencing systems:
Optimized for sequencing performance on the NovaSeq 6000 in cooperation with Illumina
Also compatible with Illumina MiSeq®, NextSeq®, HiSeq® instrument platforms