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plexWell™ WGS 24
- SKU: WGS24
$1,000.00
Out of stock
Update on plexWell™ WGS 24 (WGS24):
As of March 31, 2023, the manufacture and sale of the plexWell WGS 24 kit has been discontinued. Please contact us for early access to future products designed for whole genome sequencing or hybrid capture applications.
Description:
- Library prep for 24 gDNA samples for sequencing on the NovaSeq 6000
- 24-plex for higher-complexity human/plant/animal gDNA library prep
- Uniform >500bp insert sizing and better read balance across samples
- Kit contains all major reagents necessary to prepare Illumina-compatible NGS libraries including MagwiseTM paramagnetic beads (DNA polymerase not included)
Recommended Application:
- High-complexity genome sequencing
- Human
- Animal
- Plant
plexWell™ WGS24 Library Prep Workflow:
plexWell™ WGS24 Library Prep Chemistry:
Specifications:
- Time-to-prepare a multiplexed library from 24 samples:
- Hands-on time: 1 hr
- Total elapsed time: 3 hrs
- DNA Input: 200 ng of genomic DNA per sample (n=24)
- Expected Results:
- Library Yield: 750 – 1500 fmoles of purified multiplexed library
- Library Diversity: <6 % dupe rate when human genomic DNA is sequenced to 30X depth
- Substantial normalization: Typically <1.5-fold range (min to max) in read count across all 24 sample
- Compatibility with Illumina sequencing systems:
- Optimized for sequencing performance on the NovaSeq 6000 in cooperation with Illumina
- Also compatible with Illumina MiSeq®, NextSeq®, HiSeq® instrument platforms