Library prep for 24 gDNA samples for sequencing on the NovaSeq 6000
24-plex for higher-complexity human/plant/animal gDNA library prep
Uniform >500bp insert sizing and better read balance across samples
Kit contains all major reagents necessary to prepare Illumina-compatible NGS libraries including MagwiseTM paramagnetic beads (DNA polymerase not included)
Recommended Application:
High-complexity genome sequencing
Human
Animal
Plant
plexWell™ WGS24 Library Prep Workflow:
plexWell™ WGS24 Library Prep Chemistry:
Specifications:
Time-to-prepare a multiplexed library from 24 samples:
Hands-on time: 1 hr
Total elapsed time: 3 hrs
DNA Input: 200 ng of genomic DNA per sample (n=24)
Expected Results:
Library Yield: 750 – 1500 fmoles of purified multiplexed library
Library Diversity: <6 % dupe rate when human genomic DNA is sequenced to 30X depth
Substantial normalization: Typically <1.5-fold range (min to max) in read count across all 24 sample
Compatibility with Illumina sequencing systems:
Optimized for sequencing performance on the NovaSeq 6000 in cooperation with Illumina
Also compatible with Illumina MiSeq®, NextSeq®, HiSeq® instrument platforms