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Features and benefits:

  • End-to-end library preparation for scalable, cost-effective single-cell transcriptome analysis in a single day
  • High-efficiency workflow delivers full-length transcript coverage with highly sensitive transcript and gene detection
  • Novel auto-normalization reduces QC burden by 75%
  • Barcoding strategy enables multiplexing of up to 1,152 single cells for sequencing

      Streamlined scRNA-seq

Features and benefits

Product Highlights

Highly sensitive library preparation of full-length transcripts from low amounts of starting material

Auto-normalization ensures a highly even read distribution across samples with minimal sample QC.

Library number vs. Proportion of reads
plexWell Rapid reduces inter-sample read variability while also reducing sample QC burden by 75%.

Sensitive transcript identification and expression analysis with as little as 2 pg total RNA.

Competition vs plexWell Rapid chart

plexWell Rapid detects more genes across multiple cell types and with as little as 2 pg total RNA.

Highly reproducible performance ensures reliable results.

plexWell Rapid provides more reproducible transcript abundance results across technical replicates at ultra-low RNA input amounts.

High-quality performance with challenging samples, such as PBMCs.

plexWell Rapid delivers sensitive gene detection and highly uniform, full-length gene body coverage with challenging samples such as PBMCs.

Advantages of plate-based, full-length transcriptome sequencing

  • Complements 3’ and 5’-only droplet-based methods by expanding on cell subpopulations of interest
  • Allows for more in-depth analyses, including allele-specific expression profiling, isoform detection, and SNP and variant profiling across full-length transcripts
  • Detects more transcripts per cell, including those of lower abundance
  • Utilizes FACS instruments for cell sorting and achieves higher cell capture efficiency
  • Requires far fewer cells for successful analysis
  • Enables long-term cell storage post-sorting

Specifications

Specs Description
Compatible Sequencing Platforms Illumina MiSeq, MiniSeq, NextSeq, HiSeq, and NovaSeq instruments
Starting Material Cells and intact total RNA
Input Amount 1 to 1,000 cells
2 pg to 10 ng intact total RNA
Transcript coverage Full-length

 

plexWell Rapid Single Cell RNA Library Prep Kits include:

  • Lysis Buffer
  • Oligo Mix
  • Rapid cDNA Synthesis Buffer
  • Rapid RT
  • RNase Inhibitor
  • MAGwise
  • Sample Barcode Plates
  • 3X Coding Buffer
  • X Solution
  • Pool Barcode Reagent
  • Library Primer Mix