This product retains the batch size flexibility, normalization, and supported paired reads/clusters (4 million) of plexWell Plus 24, while significantly reducing the total time and hands-on time required to prepare libraries.
Only 90 minutes are required to prepare your libraries from start to finish.
More flexible format for different multiplex batch sizes (8 to 96)
Ideal configuration for smaller benchtop sequencers
Kit contains all major reagents necessary to prepare libraries including MagwiseTMparamagnetic beads (DNA polymerase not included)
plexWell™ Library Prep Workflow:
plexWell™ Library Prep Chemistry:
Time-to-prepare a multiplexed library from 24 samples:
Hands-on time: 35 minutes
Total elapsed time: 2.5 hours
Recommended input: 3-30 ng of genomic DNA per sample (n=24)
Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
Substantial normalization: Typically <2-fold range (min to max) in read count across all 24 samples
Compatibility with Illumina sequencing systems:
Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms