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Description:

  • More flexible format for different multiplex batch sizes (8 to 96)
  • Ideal configuration for smaller benchtop sequencers
  • Kit contains all major reagents necessary to prepare libraries including MagwiseTMparamagnetic beads (DNA polymerase not included)

Recommended Applications:

  • Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc)
  • Microbiome screening
  • Microbial whole genome sequencing
  • scRNA-seq

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

Specifications:

  • Time-to-prepare a multiplexed library from 24 samples:
    • Hands-on time: 35 minutes
    • Total elapsed time: 2.5 hours
  • Recommended input: 3-30 ng of genomic DNA per sample (n=24)
  • Expected Results:
    • Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
    • Substantial normalization: Typically <2-fold range (min to max) in read count across all 24 samples
  • Compatibility with Illumina sequencing systems:
    • Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms

Related

Caught in the Act: Tracking the Emergence and Divergence of SARS-CoV-2 through Statewide Testing and SequencingUPCOMING WEBINAR

Date:  March 23, 2022
Time: 11:00am (PDT),  2:00pm (EDT), 8:00pm (CEDT)

Learning Objectives

  • Understand the utility of full-genome viral sequence analysis for public health
  • Recognize how viral load impacts the ability to detect and sequence intact SARS-CoV-2
  • Identify the similarities and differences in host responses to SARS-CoV-2 depending on strain and immunologic history

Speakers

Frank A. Middleton, Ph.D.
Professor Director, SUNY Molecular Analysis CoreState University of New York (SUNY) Upstate Medical University

Joseph C. Mellor, Ph.D.
Co-founder and Chief Scientific Officer of seqWell Inc.