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Recommended Alternatives:

Depending on your application and needs, we recommend the following products as alternatives:

plexWell 96 or plexWell 384

  • These products maintain the same inputs and general specifications as plexWell Plus 24
  • These products require the processing of 96 samples at a time

purePlex™ DNA Library Prep Kit

  • This product retains the flexibility of processing samples in 8, 16, or 24 reaction batches and normalization features of plexWell Plus 24.
  • purePlex adds Unique Dual Indices (UDI’s), while supporting a higher number of paired reads/clusters of up to 20 million per sample.

ExpressPlex™ Library Prep Kit

  • This product retains the batch size flexibility, normalization, and supported paired reads/clusters (4 million) of plexWell Plus 24, while significantly reducing the total time and hands-on time required to prepare libraries.
  • Only 90 minutes are required to prepare your libraries from start to finish.
  • More flexible format for different multiplex batch sizes (8 to 96)
  • Ideal configuration for smaller benchtop sequencers
  • Kit contains all major reagents necessary to prepare libraries including MagwiseTMparamagnetic beads (DNA polymerase not included)

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

Specifications:

  • Time-to-prepare a multiplexed library from 24 samples:
    • Hands-on time: 35 minutes
    • Total elapsed time: 2.5 hours
  • Recommended input: 3-30 ng of genomic DNA per sample (n=24)
  • Expected Results:
    • Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
    • Substantial normalization: Typically <2-fold range (min to max) in read count across all 24 samples
  • Compatibility with Illumina sequencing systems:
    • Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms
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