$600.00

($25 per sample)

 

  • plexWell NGS multiplexed library generation kit for Illumina®
  • Assay-ready microtiter plate
  • Kit contents include all reagents necessary to prepare Illumina-compatible NGS libraries from genomic DNA
  • 24 unique barcode combinations
  • Volume-based pricing

Click Here to View plexWell Kit Comparison Chart

Category:

Description

Description:

  • Library prep for 24 gDNA samples for sequencing on the NovaSeq 6000
  • 24-plex for higher-complexity human/plant/animal gDNA library prep
  • Uniform >400bp insert sizing and better read balance across samples
  • Kit contains all reagents necessary to prepare Illumina-compatible NGS libraries included MagwiseTM paramagnetic beads
  • Volume-based pricing (save 30-40% on total lab costs for library prep)

Recommended Applications:

  • High-complexity genome sequencing
    • Human
    • Animal
    • Plant

 

plexWell Library Prep Workflow:

plexWell_WGS_workflow_template

plexWell Library Prep Chemistry:

plexWell Library Prep Chemistry

 

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Specifications:

  • Time-to-prepare a multiplexed library from 24 samples:
    • Hands-on time:  1 hr
    • Total elapsed time: 3 hrs
  • Input: 300 ng of genomic DNA per sample (n=24)
  • Output specifications:
    • Library Yield: 750 – 1500 fmoles of purified multiplexed library
    • Library Diversity: <6 % dupe rate when human genomic DNA is sequenced to 30X depth
    • Substantial normalization: Typically <1.5-fold range (min to max) in read count across all 24 sample
  • Compatibility with Illumina sequencing systems:
    • Optimized for sequencing performance on the NovaSeq 6000 in cooperation with Illumina
    • Also compatible with Illumina MiSeq®, NextSeq®, HiSeq® instrument platforms

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Resources:

plexWell WGS-24 User Guide

plexWell WGS-24 Sample Sheet Template

plexWell Kit Index List

plexWell WGS-24 Technical Data Sheet