$4,992.00

($13 per sample)

 

plexWell 384 (PW384):

  • plexWell NGS multiplexed library generation kit for Illumina®
  • Fully-skirted assay-ready 96-well plates (4 x 96)
  • 384 unique barcode combinations
  • Volume-based pricing

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Category:

Description

Description:

  • Efficient library prep for one or more 96-well plate inputs
  • Normalizes input DNA over wide input range of 3-30 ng
  • Kit contains all major reagents necessary to prepare libraries including MagwiseTM paramagnetic beads (DNA polymerase not included)
  • Volume-based pricing (save 30-40% on total lab costs)

Recommended Applications:

  • Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc)
  • Microbiome screening
  • Microbial whole genome sequencing
  • scRNA-seq
  • Low-depth whole genome/GBS

 

plexWell Library Prep Workflow:

plexWell_96_workflow_template

plexWell Library Prep Chemistry:

plexWell Library Prep Chemistry

 

Specifications:

  • Time-to-prepare a multiplexed library from 96 samples:
    • Hands-on time:       1 hr
    • Total elapsed time: 3 hrs
  • Recommended input: 3-30 ng of genomic DNA per sample (n=96)
  • Output:
    • Library Yield: 750 – 1500 fmoles of purified multiplexed library
    • Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
    • Substantial normalization: Typically <2.5-fold range (min to max) in read count across all 96 samples
  • Compatibility with Illumina sequencing systems:
    • Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms

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Resources:

PW096/PW384 User Guide 

plexWell Kit Index List

PW384 Sample Sheet Template

plexWell Technical Data Sheet