Efficient and Highly Multiplexed Whole Viral Genome Sequencing of SARS-CoV-2 Samples
Our new plexWell sample preparation method in combination with a widely-used ARTIC RT-PCR protocol greatly simplifies and increases the throughput of multiplex whole genome sequencing of SARS-CoV-2 RNA. It provides high quality results while reducing the time and cost of sequencing efforts. The method provides numerous advantages, including:
• Robust performance
• Simple workflow
• High multiplexing capacity
This cost-effective solution can provide up to 40% savings compared to other methods and reduce processing time from sample to result. The plexWell workflow is automation-friendly and compatible with a variety of platforms. plexWell enables sequencing of up to 2,304 samples in a single sequencing run and provides a simple workflow capable of meeting the high demand for COVID-19 testing.
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We need to understand the SARS-CoV-2 virus now, and NGS provides a scalable approach to quickly analyze thousands of specimens.
SARS-CoV-2 sequencing using plexWell
Generate NGS libraries for whole genome sequencing of SARS-CoV-2 isolates, combining methods for RT-PCR from the ARTIC Network for generating tiled amplicons from coronavirus RNA  along with downstream library prep with standard plexWell multiplexed NGS library prep reagents.
Streamlined workflow for SARS-CoV-2 whole-genome sequencing
Viral load range in clinical SARS-CoV-2 clinical specimens
SARS-CoV-2 viral load varies widely in nasal swab samples that are positive for COVID-19, and plexWell assays have the sensitivity to work with all but the most dilute virus-positive samples. Total nucleic acid isolated from saliva was used as the input, with the number of input viral genome copies similar to what is found in a typical nasal swab sample (~4.2 x 104 viral copies). Following RT and multiplex PCR, libraries are prepared using the plexWell kit. The libraries were then sequenced to produce full-length SARS-CoV-2 sequences.
Uniform and high SARS-CoV-2 coverage
The plexWell library prep kit delivers uniform and high sequence coverage for even low-input SARS-CoV-2-positive samples. Even at low copy numbers (160 copies), plexWell delivered 400X coverage with strong uniformity.
High SARS-CoV-2 accuracy
The plexWell library prep kit produces libraries with high mapping rates even for low-copy number SARS-CoV-2 samples.
View product description and pricing for our plexWell 384 library prep kit.
The plexWell difference
seqWell’s workflow and reagent engineering approach helps researchers harness the capacity of modern sequencing instruments to accelerate their research at scale.