seqWell Presentations at AGBT

Come see our poster presentations at AGBT:

  • Feb. 14th (Tuesday) Poster Session:
    • Poster #303 with Joe Mellor, PhD – “Phased NGS Library Generation via Tethered Synaptic Complexes”

 

  • Feb. 15th (Wednesday) Poster Session:
    • Poster #202 with Ariele Hanek, PhD – “plexWellTM: High-Throughput Normalizing Library Prep Technology for Large-Scale Multiplexed NGS Library Construction”

Deep sequencing of MTX-treated dfr1 pools by seqWell

seqWell highlighted in PLOS ONE paper:

Reverse Chemical Genetics: Comprehensive Fitness Profiling Reveals the Spectrum of Drug Target Interactions Lai H. Wong, Sunita Sinha, Julien R. Bergeron, Joseph C. Mellor, Guri Giaever, Patrick Flaherty , Corey Nislow; PLOS, Published: September 2, 2016

 

Excerpt from journal:

“Deep sequencing of MTX-treated dfr1 pools by seqWell

 

Dfr1 amplicons prepared by PCR were first purified using the Thermo Fisher Scientific PCR purification kit, according to the manufacturer’s instructions, quantified using Qubit fluorometry (Life Technologies) and diluted for sequencing library preparation. Libraries were constructed using plexWell library kit technology (seqWell, Beverly MA). In this approach, each 1+ kb pool of diverse amplicons is tagged with a pool-specific barcode via a transposase-mediated adapter addition at random locations. After this tagging, the pools of amplicons are then pooled into a single meta-pool, and subjected to a second transposase-mediated adapter addition. Fragments of this pool containing sequence from each of the two iterative adapter additions are then amplified to yield a final sequence library representing identifiable fragments from each original amplicon pool. Sequencing data is available upon request. We have deposited the raw fastq files at the NCBI SRA under the accession number SRP072709.”

Click link below for full journal article:

http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1006275